杰丁塞伯林德纳氏酵母对育成期乌苏里貉养分利用及粪便真菌菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2023.08.010

摘要/Abstract

摘要： 【目的】研究饲粮中添加杰丁塞伯林德纳氏酵母对育成期乌苏里貉营养物质消化率、氮代谢、血清生化指标及粪便真菌菌群组成的影响。【方法】选取健康的(65±5)日龄体重相近雄性乌苏里貉45只,随机分成3组,每组15只,对照组(N组)饲喂基础饲粮,试验组分别在基础饲粮中添加低(L组)和高剂量(H组)杰丁塞伯林德纳氏酵母菌,分别为每天每只2和10 mL(1×10⁹ CFU/mL)。预试期7 d,正试期30 d。于正试期最后3 d采集粪便和饲粮样品,测定各营养物质表观消化率;试验结束当天晨饲前采集血液,晨饲后采集新鲜粪便,测定血清生化指标和粪便真菌菌群组成。【结果】与N组相比,①L组乌苏里貉粗脂肪和粗蛋白质消化率显著提高(P＜0.05),L和H组乌苏里貉氮沉积和净蛋白质利用率显著升高(P＜0.05),L组乌苏里貉粪氮排出量显著降低(P＜0.05);②L和H组乌苏里貉血清天门冬氨酸氨基转移酶(AST)活性和甘油三酯(TG)水平均显著升高(P＜0.05);③H组乌苏里貉粪便真菌Chao1和ACE指数均显著降低(P＜0.05),L和H组乌苏里貉粪便曲霉菌属(Aspergillus)和篮状菌属(Talaromyces)的相对丰度均显著降低(P＜0.05),H组乌苏里貉粪便芽枝霉属(Cladosporium)的相对丰度显著降低(P＜0.05)。【结论】饲粮中添加杰丁塞伯林德纳氏酵母可提高育成期乌苏里貉的营养物质消化率、氮代谢,降低粪便有害真菌的相对丰度,综合考虑,其适宜的添加量为每天每只2 mL(1×10⁹ CFU/mL)。

关键词: 杰丁塞伯林德纳氏酵母; 乌苏里貉; 营养物质消化率; 氮代谢; 真菌菌群结构

Abstract: 【Objective】 This experiment was conducted to investigate the effects of dietary supplementation of Cyberlindnera jadinii on nutrient digestibility,nitrogen metabolism,serum biochemical indexes and composition of fecal fungal community in growing raccoon dogs.【Method】 Forty-five healthy male raccoon dog with similar body weight at (60±5) day of age were randomly assigned to three treatment groups,with 15 raccoon dogs per group.Raccoon dogs in control group (N group) were fed a basal diet,raccoon dogs in experiment groups were fed the basal diet supplemented with low (L group) and high doses (H group) of Cyberlindnera jadinii,2 and 10 mL (1×10⁹ CFU/mL) per animal every day,respectively.A 7-day pretest period preceded a formal test period of 30 days.Manure and rations samples were collected in the last 3 days of the trial period to determine the nutrient apparent digestibility.Blood was collected before the morning feeding and fresh manure was collected after the morning feeding at the end of the trial period to determine the serum biochemical and fecal fungi composition.【Result】 Compared with N group,①the digestibility of ether extract and crude protein of raccoon dogs in L group were significantly increased (P＜0.05).The nitrogen retention and net protein utilization of raccoon dogs in L and H groups were significantly increased (P＜0.05).The fecal nitrogen excretion of raccoon dogs in L group was significantly decreased (P＜0.05).②Serum aspartate aminotransferase activity(AST) and triglycerides(TG) levels of raccoon dogs in L and H groups were significantly increased (P＜0.05).③The Chao1 and ACE indices of fecal fungi of raccoon dogs in H group were significantly decreased (P＜0.05).The relative abundances of Aspergillus and Talaromyces in the feces of raccoon dogs in L and H groups were significantly decreased (P＜0.05).The relative abundance of Cladosporium in the feces of raccoon dogs in H group was significantly decreased (P＜0.05).【Conclusion】 Dietary supplementation of Cyberlindnera jadinii could improve nutrient digestibility,nitrogen metabolism and reduce the relative abundance of harmful fungi in the feces of raccoon dogs.2 mL (1×10⁹ CFU/mL) per animal every day might be the most suitable addition level under the experimental conditions.

Key words: Cyberlindnera jadinii; raccoon dogs; nutrient digestibility; nitrogen metabolism; fungi composition

中图分类号:

S816.7

赵德辉, 马翠柳, 吴炎, 刘可园, 张博睿, 张海华, 刘晗璐. 杰丁塞伯林德纳氏酵母对育成期乌苏里貉养分利用及粪便真菌菌群结构的影响[J]. 中国畜牧兽医, 2023, 50(8): 3123-3132.

ZHAO Dehui, MA Cuiliu, WU Yan, LIU Keyuan, ZHANG Borui, ZHANG Haihua, LIU Hanlu. The Effects of Cyberlindnera jadinii on Nutrient Utilization,Serum Biochemical Indexes and Fecal Fungi Composition in Growing Raccoon Dogs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3123-3132.

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