地菍总黄酮对高脂饮食诱导非酒精性脂肪肝大鼠脂代谢的影响

doi:10.16431/j.cnki.1671-7236.2023.08.042

摘要/Abstract

摘要： 【目的】探讨地菍总黄酮对高脂饮食诱导非酒精性脂肪肝(NAFLD)模型大鼠脂代谢的影响。【方法】将60只SPF级雄性大鼠随机分成空白组、模型组、奥利司他组以及地菍总黄酮高、中、低剂量组,每组10只。空白组大鼠饲喂基础饲粮,其余各组饲喂高脂饲粮,奥利他司组给予60 mg/kg奥利他司,地菍总黄酮高、中、低剂量组分别给予750、500、330 mg/kg地菍总黄酮,每天以0.1 mL/10 g灌胃体积给予相应的药物,连续给药42 d,空白组、模型组给予等体积蒸馏水。记录各组大鼠的一般状况,给药结束后检测各组大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及脂联素(ADP)、瘦素(LEP)水平,并统计胰岛素敏感指数(ISI)、胰岛素抵抗指数(HOMA-IR);检测肝脏组织中甘油三酯(TG)、总胆固醇(TC)含量;油红O染色观察肝脏病理变化;用实时荧光定量PCR和Western blotting方法分别检测各组大鼠肝脏中过氧化物酶增殖物激活受体γ(PPARγ)、脂联素受体2(AdipoR2)、p38的mRNA和蛋白表达量。【结果】与空白组相比,模型组大鼠血清ALT、AST活性及LEP、FINS、FBG、HOMA-IR水平极显著或显著升高(P＜0.01;P＜0.05),ADP、ISI水平极显著下降(P＜0.01),肝脏中脂肪沉积较多,成功构建NAFLD大鼠模型。与模型组相比,奥利司他组、地菍总黄酮高剂量组大鼠血清ALT、AST活性以及LEP、FINS、FBG、HOMA-IR水平显著或极显著下降(P＜0.05;P＜0.01),ADP、ISI水平极显著升高(P＜0.01);奥利司他组及地菍总黄酮高、中、低剂量组肝脏TC和TG含量均极显著或显著降低(P＜0.01;P＜0.05),肝脏中脂肪沉积较少,且PPARγ、AdipoR2、p38的mRNA和蛋白水平均极显著升高(P＜0.01)。【结论】地菍总黄酮可明显改善高脂饮食诱导大鼠的NAFLD,其作用机制可能与其参与调节糖脂代谢、改善胰岛素抵抗、调控PPARγ/AdipoR2/p38/信号通路相关。

关键词: 地菍; 总黄酮; 非酒精性脂肪肝; 脂代谢

Abstract: 【Objective】 This study was conducted to explore the effect of total flavonoids from Melastoma dodecandrum Lour.on lipid metabolism in rats with high fat diet induced nonalcoholic fatty liver disease (NAFLD).【Method】 Sixty SPF male rats were randomly divided into blank group,model group,orlistat group,high,medium and low dose of total flavonoids from Melastoma dodecandrum Lour.groups,with 10 rats in each group.The rats in blank group were fed a basal diet,and the other groups were fed a high fat diet.Orlistat group was given 60 mg/kg orlistat,while high,medium and low dose groups were given 750,500 and 330 mg/kg total flavonoids from Melastoma dodecandrum Lour.,respectively.The corresponding drugs were given by intragastric administration at the volume of 0.1 mL/10 g every day for 42 days.The blank and model group were given equal volume of distilled water.The general status of rats in each group was recorded,and fasting blood glucose (FBG),fasting insulin (FINS),the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the levels of adiponectin (ADP) and leptin (LEP) were detected after administration.Insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) were analyzed.The contents of triglyceride (TG) and total cholesterol (TC) in liver were detected.Oil red O staining was used to observe the pathological changes of liver.The mRNA and protein expressions of peroxidase proliferator-activated receptor γ (PPARγ),adiponectin receptor 2 (AdipoR2) and p38 in liver of rats in each group were detected by Real-time quantitative PCR and Western blotting,respectively.【Result】 Compared with blank group, the activities of ALT and AST and the levels of LEP, FINS, FBG and HOMA-IR of serum in model group were extremely significantly or significantly increased (P＜0.01 or P＜0.05), the levels of ADP and ISI were extremely significantly decreased (P＜0.01), and more fat was deposited in the liver. The NAFLD model of rat was successfully established. Compared with model group,the activities of ALT and AST in serum and the levels of LEP,FINS,FBG and HOMA-IR in orlistat and high dose of total flavonoids from Melastoma dodecandrum Lour.groups were significantly or extremely significantly decreased (P<0.05 or P<0.01),ADP and ISI levels were extremely significantly increased (P<0.01).The contents of TC and TG in liver of orlistat and high,medium and low dose of total flavonoids from Melastoma dodecandrum Lour.groups were extremely significantly or significantly decreased (P<0.01 or P<0.05),there was less fat deposition in liver,and the mRNA and protein levels of PPARγ,AdipoR2 and p38 were extremely significantly increased (P<0.01).【Conclusion】 Total flavonoids from Melastoma dodecandrum Lour.could significantly improve NAFLD in rats induced by high fat diet,and its mechanism might be related to its involvement in regulating glucolipid metabolism,improving insulin resistance,and regulating PPARγ/AdipoR2/p38 signaling pathway.

Key words: Melastoma dodecandrum Lour.; total flavonoids; nonalcoholic fatty liver disease; lipid metabolism

中图分类号:

R285.5

唐雨菲, 周敬凯, 莫烨云, 朱盼, 杨秋莉, 李笑笑, 李丽. 地菍总黄酮对高脂饮食诱导非酒精性脂肪肝大鼠脂代谢的影响[J]. 中国畜牧兽医, 2023, 50(8): 3438-3447.

TANG Yufei, ZHOU Jingkai, MO Yeyun, ZHU Pan, YANG Qiuli, LI Xiaoxiao, LI Li. Effect of Total Flavonoids from Melastoma dodecandrum Lour.on Lipid Metabolism of Nonalcoholic Fatty Liver Rats induced by High-fat Diet[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3438-3447.

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