地菍总黄酮对胰岛素抵抗小鼠的干预作用及机制研究

doi:10.16431/j.cnki.1671-7236.2020.09.039

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (9): 3030-3037.doi: 10.16431/j.cnki.1671-7236.2020.09.039

• 基础兽医 • 上一篇

地菍总黄酮对胰岛素抵抗小鼠的干预作用及机制研究

周敬凯¹, 黄馨慧², 刘舒凌¹, 翁竞玉¹, 朱盼¹, 李丽¹

1. 广西中医药大学药学院, 南宁 530001;
2. 广西国际壮医医院药学部, 南宁 530201

收稿日期:2020-03-17 出版日期:2020-09-20 发布日期:2020-09-16
通讯作者: 李丽 E-mail:lilygxnn@163.com
作者简介:周敬凯(1995-),男,广西百色人,硕士生,研究方向:中药药理,E-mail:530437252@qq.com
基金资助:
广西一流学科建设开放课题（2019XK082）；广西壮瑶药重点实验室立项项目（GXZYZZ2019-8）；广西研究生教育创新计划资助项目（YCSY2020042）；广西中医药大学横向课题（H2013001）；壮瑶药协同创新中心（桂教科研[2013]20号）；广西重点学科（壮药学）（桂教科研[2013]16号）；广西自然科学基金项目（2013GXNSFAA019134）

Study on the Intervention Effect and Mechanism on Insulin Resistant Mice of Total Flavone from Melastoma dodecandrum Lour.

ZHOU Jingkai¹, HUANG Xinhui², LIU Shulin¹, WENG Jingyu¹, ZHU Pan¹, LI Li¹

1. College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China;
2. Pharmaceutical Department of Guangxi International Zhuang Medical Hospital, Nanning 530201, China

Received:2020-03-17 Online:2020-09-20 Published:2020-09-16

摘要/Abstract

摘要： 本研究旨在探讨地菍总黄酮（TFMD）对胰岛素抵抗（IR）小鼠的干预作用及其机制。试验以灌胃高脂乳剂建立胰岛素抵抗小鼠模型，同时灌胃给予地菍总黄酮混悬液（高剂量（600 mg/kg）、中剂量（300 mg/kg）及低剂量（150 mg/kg））进行治疗，治疗周期为30 d。试验结束后测定小鼠血清中空腹血清胰岛素（FINS）、空腹血糖（FBG）、胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（ISI）、血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白（LDL）及高密度脂蛋白（HDL）水平；利用实时荧光定量PCR法检测各组小鼠肝脏中胰岛素受体（InsR）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）及骨骼肌中葡萄糖转运体4（GLUT4）的mRNA表达水平；利用免疫组化技术检测各组小鼠肝脏中InsR、PPAR-γ和骨骼肌中GLUT4的蛋白表达水平。结果显示，与模型组相比，地菍总黄酮能降低IR小鼠的体重，降低血清FBG、FINS及HOMA-IR水平，升高ISI水平（P<0.01，P<0.05）；降低血清TG、TC及LDL含量，升高HDL含量（P<0.01，P<0.05）；同时提高IR小鼠肝脏中InsR、PPAR-γ及骨骼肌中GLUT4的mRNA表达量，提高小鼠肝脏InsR、PPAR-γ及骨骼肌中GLUT4的蛋白表达水平（P<0.01，P<0.05）。以上试验结果证实，地菍总黄酮能有效缓解小鼠试验性胰岛素抵抗症状，该活性与调节糖脂代谢、增强胰岛素敏感性有关。

关键词: 地菍; 总黄酮; 胰岛素抵抗; 糖代谢

Abstract: This experiment was conducted to explore the intervention effect and mechanism on insulin resistance (IR) mice of total flavonoids from Melastoma dodecandrum Lour.(TFMD).The model of IR mice was established by intragastric administration of high-fat emulsion,while 600,300 and 150 mg/kg TFMD were administered once daily for 30 days.After the end of the experiment the mices' fasting blood glucose (FBG),fasting serum insulin (FINS),serum total cholesterol(TC),triglyceride (TG),high density lipoprotein(HDL) were determined.The mRNA expression level of liver insulin receptor(InsR),fat peroxisome proliferator-activated receptor-γ(PPAR-γ),and glucose transporter 4 gene (GLUT4) in skeletal muscle were detected by Real-time quantative PCR,and the protein levels were detected by immunohistoche mical method.The results showed that TDMF could reduce the body weight of IR mice,decrease the level of serum FBG,FINS and HOMA-IR,increase the level of ISI,decrease the content of serum TG,TC and LDL,and increase the content of HDL(P<0.01,P<0.05);And the mRNA expression of INSR,PPAR-γ in liver and GLUT4 in skeletal muscle of IR mice were increased(P<0.01,P<0.05),and the protein expression level of InsR,PPAR-γ in liver and GLUT4 in skeletal muscle of IR mice were increased(P<0.01,P<0.05).The results confirmed that the TFMD could relieve experimental insulin resistance in mice,and the activity was related to the regulation of glucose and lipid metabolism and the enhancement of insulin sensitivity.

Key words: Melastoma dodecandrum Lour.; total flavonoids; insulin resistance; glucose metabolism

中图分类号:

S853.74

周敬凯, 黄馨慧, 刘舒凌, 翁竞玉, 朱盼, 李丽. 地菍总黄酮对胰岛素抵抗小鼠的干预作用及机制研究[J]. 中国畜牧兽医, 2020, 47(9): 3030-3037.

ZHOU Jingkai, HUANG Xinhui, LIU Shulin, WENG Jingyu, ZHU Pan, LI Li. Study on the Intervention Effect and Mechanism on Insulin Resistant Mice of Total Flavone from Melastoma dodecandrum Lour.[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 3030-3037.

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地菍总黄酮对胰岛素抵抗小鼠的干预作用及机制研究

Study on the Intervention Effect and Mechanism on Insulin Resistant Mice of Total Flavone from Melastoma dodecandrum Lour.

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