地菍粗多糖对高脂饮食诱导肥胖小鼠脂代谢的影响

doi:10.16431/j.cnki.1671-7236.2022.01.013

摘要/Abstract

摘要： [目的] 研究地菍粗多糖对高脂饮食诱导肥胖小鼠脂代谢的影响。[方法] 将小鼠随机分为6组：空白组、模型组、阳性组（0.025 mg/g奥利司他）及高、中、低剂量地菍粗多糖（1.2、0.6和0.3 mg/g）处理组，每组12只。空白组小鼠喂食普通维持饲料，其余各组小鼠喂食高脂饲料，空白组、模型组给予生理盐水，阳性组给予0.025 mg/g奥利司他，高、中、低剂量地菍粗多糖组分别给予1.2、0.6和0.3 mg/g地菍粗多糖，每天灌胃1次，连续给药30 d。给药结束，称量小鼠体重、附睾及肾脏周围脂肪和肝脏组织的重量；HE染色法观察脂肪和肝脏病理变化；生化法检测血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）含量及肝脏TC、TG含量；实时荧光定量PCR法测定肝脏中乙酰辅酶A羧化酶1（ACC1）基因相对表达量。[结果] 与模型组相比，高、中剂量地菍粗多糖组小鼠体重、Lee's指数、摄食量、肝脏及附睾和肾脏周围脂肪的重量均显著降低（P<0.05），低剂量地菍粗多糖组小鼠体重、脂肪重量均无显著变化（P>0.05），阳性组小鼠摄食量、肝脏重量均无显著变化（P>0.05）；阳性组、高剂量地菍粗多糖组小鼠的脂肪指数均显著降低（P<0.05）；阳性组、高剂量地菍粗多糖组小鼠血清TC、TG、LDL-C及肝脏TC、TG含量均显著降低（P<0.05），血清HDL-C含量差异不显著（P>0.05）；中剂量地菍粗多糖组小鼠血清TC、TG及肝脏TC含量均显著降低（P<0.05）。阳性组及高、中、低剂量地菍粗多糖组小鼠脂肪细胞均不同程度变小，肝细胞内脂滴小空泡明显减少，且ACC1基因的相对表达量均显著下调（P<0.05），分别降低了20%、42%、15%和11%。[结论] 地菍粗多糖可通过调节高脂饮食诱导肥胖小鼠的脂代谢水平，从而干预肥胖的发生。

关键词: 地菍; 粗多糖; 肥胖; 脂代谢

Abstract: [Objective] This study was aimed to investigate the effect of crude polysaccharide from Melastoma dodecandrum Lour. on lipid metabolism in obese mice induced by high-fat diet. [Method] The mice were randomly divided into 6 groups, including blank group, model group, positive group (0.025 mg/g orlistat), high, medium and low dose of crude polysaccharide from Melastoma dodecandrum Lour. groups (1.2, 0.6 and 0.3 mg/g), with 12 mice in each group. The mice in blank group were fed with normal maintenance diet, the mice in the other groups were fed with high-fat diet, the mice in blank group and model group were given normal saline, the mice in positive group were given 0.025 mg/g orlistat, and the mice in high, medium and low dose groups were given 1.2, 0.6 and 0.3 mg/g crude polysaccharides of Melastoma dodecandrum Lour., once a day for 30 days. The weight of mice, epididymis, fat around kidney and liver tissuea were measured. The pathological changes of fat and liver were observed by HE staining. Serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) and liver TC and TG were detected by biochemical method. The relative expression of acetyl CoA carboxylase 1 (ACC1) gene in liver was determined by Real-time quantitative PCR. [Result] Compared with model group, the body weight, Lee's index, food intake, the weight of liver, epididymis fat and kidney fat were significantly decreased in the high and medium dose groups (P<0.05). The body weight and fat weight of mice in low dose group had no significant change (P>0.05), the food intake and liver weight of mice in positive group had no significant change (P>0.05). The fat index of mice in positive and high dose groups was significantly decreased (P<0.05). The contents of serum TC, TG, LDL-C and liver TC, TG in positive and high dose groups were significantly decreased (P<0.05), but there was no significant difference of serum HDL-C (P>0.05). The contents of TC, TG in serum and TC in liver were significantly decreased in middle dose group (P<0.05). In positive group, high, medium and low dose groups, the adipocytes were reduced in varying degrees, and the lipid droplets and vacuoles in hepatocytes were significantly reduced (P<0.05), and the relative expression of ACC1 gene was significantly down regulated (P<0.05) by 20%, 42%, 15% and 11%, respectively. [Conclusion] The crude polysaccharide of Melastoma dodecandrum Lour. could intervene the occurrence of obesity by regulating the level of lipid metabolism in obese mice induced by high-fat diet.

Key words: Melastoma dodecandrum Lour.; crude polysaccharide; obesity; lipid metabolism

中图分类号:

R285.5

李丽, 朱盼, 郭兴兰, 潘炳强, 周敬凯, 陈俊. 地菍粗多糖对高脂饮食诱导肥胖小鼠脂代谢的影响[J]. 中国畜牧兽医, 2022, 49(1): 122-130.

LI Li, ZHU Pan, GUO Xinglan, PAN Bingqiang, ZHOU Jingkai, CHEN Jun. Effect of Crude Polysaccharide from Melastoma dodecandrum Lour. on Lipid Metabolism of Obese Mice Induced by High-fat Diet[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 122-130.

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