›› 2012, Vol. 39 ›› Issue (4): 139-142.

• 遗传繁育 • 上一篇    下一篇

Calsarcin-2基因第1内含子遗传多态性分析

吴眩, 许厚强, 乜玉丽, 陈祥, 张勇   

  1. 贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室, 贵州省动物遗传育种与繁殖人才基地,贵州贵阳 550025
  • 收稿日期:2011-09-01 修回日期:1900-01-01 出版日期:2012-04-20 发布日期:2012-04-20
  • 通讯作者: 许厚强

Genetic Polymorphism Analysis on Intron 1 of Calsarcin-2 in Porcine

WU Xuan, XU Hou-qiang, NIE Yu-li, CHEN Xiang, ZHANG Yong   

  1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region,Guizhou University, Ministry of Education;Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction; Guizhou Talent Base of Animal Genetics, Breeding and Reproduction,Guiyang 550025,China
  • Received:2011-09-01 Revised:1900-01-01 Online:2012-04-20 Published:2012-04-20

摘要: 以大约克猪、长白猪、杜洛克猪和贵州白香猪杂交及回交的后代为研究对象,根据NCBI公布的序列设计了4对引物,采用测序、PCR-SSCP技术对Calsarcin-2基因第1内含子进行了单核苷酸多态性检测和基因型分析,探讨Calsarcin-2基因第1内含子多态性与肉质性状的关系。结果发现2个SNPs(T613C、C936G),群体遗传学分析表明,在所检测的各多态位点上,不同群体间不同基因型的分布都存在着极显著差异(P<0.01),结合品种特性分析表明,613及936位点所检测的不同基因型和肉质可能具有一定的相关性。

关键词: 猪; Calsarcin-2基因; 肉质; PCR-SSCP

Abstract: In this study, the crossbreed and backcross of Yorkshire, Landrace, Duroc and Guizhou Bai Xiang pig were used to investigate the effect of Calsarcin-2 gene on meat quality. Four pairs of primers for intron 1 of Calsarcin-2 gene were designed according to pig sequences from GenBank. Polymorphisms were detected by PCR-SSCP method. SNPs were detected by DNA sequencing.2 SNPs(T613C,C936G) were identified. Population genetics analysis showed that the distribution of genotypes among five species were extremely significant difference (P<0.01). Association of polymorphisms with breed characteristics indicated that the mutations of 613 and 936 sites may be related to meat quality.

Key words: pig; Calsarcin-2 gene; meat quality; PCR-SSCP

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