›› 2011, Vol. 38 ›› Issue (5): 217-219.

• 经验交流 • 上一篇    下一篇

60株大肠杆菌的分离与致病性鉴定

黄宗梅1, 陈红英1, 崔沛2, 韩玉林3, 李新生1   

  1. 1. 河南农业大学牧医工程学院, 河南郑州 450002;2. 河南省动物疫病预防控制中心, 河南郑州 450000;3. 焦作市畜牧局, 河南焦作 454150
  • 收稿日期:2010-10-08 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 通讯作者: 李新生

Isolation and Identification of 60 Pathogenic Escherichia coli Strains

HUANG Zong-mei1, CHEN Hong-ying1, CUI Pei2, HAN Yu-lin3, LI Xin-sheng1   

  1. 1. College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;2. Henan Province Veterinary Preventive and Treatment Station,Zhengzhou 450000,China;3. Jiaozuo Husbandry Bureau,Jiaozuo 454150, China
  • Received:2010-10-08 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

摘要: 大肠杆菌(Escherichia coli,E.coli)是鸡肠道常在菌,部分菌株具有致病性。鸡大肠杆菌病就是由大肠杆菌中的某些致病性菌株引起的鸡感染性疾病。致病性大肠杆菌均携带毒力岛基因ChuA。为鉴定分离自临床病例的鸡大肠杆菌的致病性,本试验采用PCR法检测ChuA基因进行分子生物学鉴定,60株大肠杆菌中有31株属于致病性大肠杆菌,总阳性率为51.67%。

关键词: 鸡; 致病性大肠杆菌; 分离鉴定

Abstract: Escherichia coli (E.coli) is often in chicken intestinal. Only some strains are pathogenic for chicken. Chicken Escherichia coli disease is caused by these pathogenic E.coli strains,and they have pathogenicity island genes ChuA. In order to identify 60 E.coli strains isolated from diseased chicken,ChuA gene was tested by PCR. 31 E.coli strains are pathogenic. The total positive rate is 51.67%.

Key words: chicken; pathogenic Escherichia coli; isolation and identification

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