蕨麻猪生长激素基因的同源性和多态性分析

›› 2010, Vol. 37 ›› Issue (4): 98-102.

蕨麻猪生长激素基因的同源性和多态性分析

李军成^1，2，路彩霞²，景志忠¹

（1.中国农业科学院兰州兽医研究所，家畜疫病病原生物学国家重点实验室，农业部兽医公共卫生重点实验室，甘肃省动物寄生虫病重点实验室，兰州 730046； 2.广西农业职业技术学院，南宁 530007)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20
通讯作者: 景志忠

Homology and Polymorphism Analysis of Juema Swine Growth Hormone Gene

LI Jun-cheng^1，2，LU Cai-xia²，JING Zhi-zhong¹

(1.Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China; 2.Guangxi Agricultural Vocation-Technical College, Nanning 530007, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
Contact: JING Zhi-zhong

摘要/Abstract

摘要： 为了探索蕨麻猪的遗传多样性，对蕨麻猪生长激素基因序列的多态性进行研究，并选择GenBank中14条序列作为比较研究对象；首次成功克隆了7条蕨麻猪生长激素基因，测出5条蕨麻猪生长激素基因DNA序列，其多态性是内含子1有1个转换和1个颠换；内含子2有2个转换。说明蕨麻猪之间的差异可能与内含子的控制有关。蕨麻猪与其他14种猪之间在编码区和非编码区共检测到核苷酸的变异为155个，内含子有119个，外显子有28个，5′端有8个。其中，颠换104个，转换14个，缺失23个，插入14个。出现碱基取代的位点中，大多数是颠换型突变，颠换与转换之比为7.43∶1，存在颠换偏倚现象。对蕨麻猪生长激素基因同源性百分数和趋异数分析，结果发现，蕨麻猪与小型猪相比同源性较近，蕨麻猪与大型猪同源性较远。蕨麻猪等15 种猪生长激素基因序列树状图分析，结果表明，蕨麻猪与Vize猪有较远的亲缘关系；蕨麻猪与贵州榕江香猪亲缘关系最近。

关键词: 蕨麻猪; 生长激素基因; 同源性; 多态性

Abstract:

For exploring genetic diversity of Juema pig, researched on the polymorphism of growth hormone gene order of Juema pig, and selected sequences in 14 kinds of pig from GenBank. Full sequence of growth hormone gene of 7 Juema pigs had been cloned successfully for the first time, from which 5 Juema pigs were sequenced respectively, whose polymorphism is intron 1 contains 1 commutation and 1 transversion, intron 2 contains 2 transversions, explain that the difference between Juma pigs may be connected with controlling of intron. The variation of 155 nucleotide all were detected in coding and noncoding region by comparing Juema pig with other 14 breeds, and 119 out of them were in intron regions and 28 out of them were in exon regions, with 104 transversions, 14 commutations, 23 absences, 14 inserts among them. Among substitutive sites of basic group, most was transversion mutations; the comparision between transversion and commutations was 7.43∶1, which exists transversion bias phenomenon. The percentage and divergence number of Juema pig’s growth hormone gene homology analyse showed that homology was nearer by comparing Juema pig with other minitype pig, homology of Juema pig and other large pig was farer. Dendrogram of growth hormone gene sequence in Juema pig etc. of 15 kinds analyse, which indicated Juema pig having far distant genetic relationship with the Vize pig; Juema pig and Rongjiang pig’s genetic relationships were nearest.

Key words: Juema swine; growth hormone gene; homology; polymorphism

中图分类号:

李军成;路彩霞;景志忠. 蕨麻猪生长激素基因的同源性和多态性分析[J]. , 2010, 37(4): 98-102.

LI Jun-cheng;LU Cai-xia;JING Zhi-zhong. Homology and Polymorphism Analysis of Juema Swine Growth Hormone Gene[J]. , 2010, 37(4): 98-102.

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