›› 2010, Vol. 37 ›› Issue (4): 94-97.

• 生物技术 • 上一篇    下一篇

沙门氏菌PCR检测方法的建立

许会会,雷连成,谢芳,杜涛峰,韩文瑜   

  1. (吉林大学畜牧兽医学院, 长春 130062)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20
  • 通讯作者: 雷连成

Development of Polymerase Chain Methods for Salmonella

XU Hui-hui, LEI Lian-cheng, XIE Fang, DU Tao-feng, HAN Wen-yu   

  1. (College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: LEI Lian-cheng

摘要: 据GenBank沙门氏菌侵袭蛋白A(invA)基因序列设计引物,扩增特异的202 bp核苷酸片段,经过优化PCR扩增条件,建立了沙门氏菌特异、敏感和快速的PCR检测方法。特异性试验结果表明,从沙门氏菌参考菌株中均能扩增出特异性的核苷酸片段,大肠杆菌、巴氏杆菌、金黄色葡萄菌、志贺氏菌、蜡样芽孢杆菌、变形杆菌、绿脓杆菌的扩增结果均为阴性。敏感性试验结果表明,采用简便的直接煮沸裂解法制备样品DNA,该方法的敏感性可达2.43×103 CFU/mL。

关键词: 沙门氏菌; PCR; 检测

Abstract: According to GenBank Salmonella invasion protein A (invA) gene sequences, a pair of primers were designed for amplification of specific nucleic acid fragment of 202 bp, after optimizing the PCR amplification conditions, established a Salmonella-specific, sensitive and rapid PCR detection method. Specificity test results showed that Salmonella strains are able to amplify the specific nucleic acid fragment, the results of E.coli, Pasteurella multocida, Staphylococcus aureus, Shigella, Bacillus cereus, Proteus mirabilis, Pseudomonas aeruginosa were negative. Sensitivity test results showed that the sensitivity of the method can reach 2.43×103 CFU/mL.

Key words: Salmonella; PCR; detection

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