›› 2010, Vol. 37 ›› Issue (3): 41-44.

• 生理生化 • 上一篇    下一篇

文昌鸡肌肉脂肪酸组分的气相色谱—质谱联合分析

常国斌1, 雷黎立1, 王克华2, 王存波1, 陈国宏1   

  1. (1. 扬州大学动物科学与科技学院, 扬州 225009; 2.中国农业科学院家禽研究所, 扬州 225003)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 陈国宏

GC-MS Analysis on the Components of Fatty Acids in the Muscle of Wenchang Chicken

CHANG Guo-bin1, LEI Li-li1, WANG Ke-hua2, WANG Cun-bo1, CHEN Guo-hong1   

  1. (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009,China; 2.Institute of Poultry, Chinese Academy of Agricultural Sciences, Yangzhou 225003,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: CHEN Guo-hong

摘要: 应用气相色谱—质谱联合技术对文昌鸡肌肉脂肪酸组分进行分析,并以面积归一法,计算各脂肪酸的相对百分比含量。结果表明,文昌鸡肌肉中共分离和鉴定出16种脂肪酸,其中饱和脂肪酸7种,包括n-十二烷酸、n-十四烷酸、十五烷酸、n-十六烷酸、十七烷酸、n-十八烷酸和二十烷酸,其含量分别为0.75%、1.23%、0.43%、23.86%、2.04%、11.18%、0.13%,占总脂肪酸的39.63%;不饱和脂肪酸9种,包括11-十四碳烯酸、9-十六碳烯酸、9-十八碳烯酸、9,12-十八碳二烯酸、6,9,12-十八碳三烯酸、11-二十碳烯酸、10,13-二十碳二烯酸、7,10,13-二十碳三烯酸及5,8,11,14-二十碳四烯酸,其含量分别为0.15%、1.79%、25.84%、21.99%、0. 48%、0.32%、0.45%、0.61%、8.75%,占总脂肪酸含量的60.37%。多不饱和脂肪酸占总脂肪酸含量的32.29%,而必需脂肪酸占总脂肪酸含量的31.23%。这一结果为家禽肌肉中脂肪酸成分分析及营养价值的评定提供了基础依据。

关键词: 文昌鸡; 脂肪酸; 气相色谱—质谱

Abstract: The components of fatty acids in Wenchang chicken were analyzed by gas chromatogrphy-mass spectrometry technology(GC-MS),and the relative content of each fatty acid were calculated. 16 kinds of fatty acids were seperated from Wenchang chicken.Among them,7 kinds of fatty acids,including lauric anid,myristic acid,pentadecanoic acid,palmitic acid,margaric,stearic acid and eicosanoic,were saturated fatty acids.The content of those 7 fatty acids were .75%,1.23%,0.43%,23.86%, 2.04%, 11.18%,0.13%,accounts for 39.63% of the total fatty acid.9 kinds of fatty acids,including 11-teteadecenoic acid,9-hexadecenoic acid,9-vaccenic acid,9,12-octadecadienoic acid,6,9,12-octadecatrienoic acid,11-eicosenoic acid,10,13-eicosenoic,7,10,13-eicosatriennoic and 5,8,11,14-arachidonic acid,were unsaturated fatty acids.The contens of 9 kinds of fatty acids were 0.15%,1.79%,25.84%,21.99%,0. 48%,0.32%,0.45%,0.61%,8.75%,accounts for 60.37% of the total fatty acid. The polyunsaturates fatty acids accounted for 32.29% of the total fatty acids and the essential fatty acids accounted for 31.23%.The results provided the basis for analysis on fatty acid acid component and evaluation on the nutritive value.

Key words: Wenchang chicken; fatty acids; GC-MS

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