不同月龄滩羊肌肉组织转录组比较及脂肪酸分析

doi:10.16431/j.cnki.1671-7236.2023.12.015

摘要/Abstract

摘要： 【目的】比较4、9月龄滩羊体脂肪代谢差异表达基因,挖掘影响滩羊不同肌肉组织脂肪代谢的基因。【方法】分别采集4、9月龄滩羊背最长肌和股二头肌,采用Illumian软件进行转录组测序分析,并对背最长肌和股二头肌中的脂肪与脂肪酸含量进行分析。【结果】与4月龄滩羊相比,9月龄滩羊股二头肌脂肪含量极显著下降(P＜0.01),背最长肌脂肪含量极显著上升(P＜0.01),油酸等不饱和脂肪酸含量升高,肉豆蔻酸、棕榈酸等饱和脂肪酸含量下降。转录组测序结果显示,滩羊股二头肌中共筛选到893个差异表达基因(上调435个,下调458个);背最长肌中共筛选到809个差异表达基因(上调544个,下调265个)。GO功能富集分析表明,滩羊股二头肌中差异表达基因显著富集到327个条目,与脂肪沉积相关的有TCA循环、呼吸链复合体、氢离子跨膜转运蛋白活性等;背最长肌中差异表达基因显著富集到28个条目,与脂肪沉积相关的有有机酸分解代谢、脂肪酸代谢、细胞外空间等。KEGG通路注释结果显示,滩羊股二头肌中差异表达基因显著富集到25条通路,其中参与脂肪代谢的有9条,如TCA循环、丙酮酸代谢等;背最长肌中差异表达基因显著富集到8条通路,其中参与脂肪沉积的有2条,分别为缬氨酸、亮氨酸和异亮氨酸降解与谷胱甘肽代谢。【结论】通过对脂肪代谢相关通路基因进行筛选发现,股二头肌中MDH2、PPARGC1A基因及背最长肌中GSTK1、MCCC2基因可能与9月龄滩羊肌肉脂肪代谢相关。研究结果为进一步探究不同生长阶段滩羊肌肉组织脂肪沉积提供理论依据。

关键词: 滩羊; 转录组; 脂肪酸; 背最长肌; 股二头肌

Abstract: 【Objective】 The differentially expressed genes of fat metabolism in 4- and 9-month-old Tan sheep were compared in this study,and the genes affecting fat metabolism in different muscle tissues of Tan sheep were excavated.【Method】 Longissimus dorsi muscle and biceps femoris muscle of 4- and 9-month-old Tan sheep were collected,the transcriptomic sequencing was performed by Illumian software,and the fat and fatty acid contents of longissimus dorsi muscle and biceps femoris muscle were analyzed.【Result】 Compared with 4-month-old Tan sheep,the fat content in biceps femoris muscle of 9-month-old Tan sheep was significantly decreased (P＜0.01),the fat content in longissimus dorsi muscle was significantly increased (P＜0.01),the unsaturated fatty acid contents such as oleic acid were increased,and the saturated fatty acid contents such as myristic acid and palmitic acid were decreased.Transcriptome sequencing results showed that a total of 893 differentially expressed genes (435 up-regulated and 458 down-regulated) were screened in biceps femoris muscle,and 809 differentially expressed genes (544 up-regulated and 265 down-regulated) were screened in longissimus dorsi muscle.GO function enrichment analysis showed that the differentially expressed genes in biceps femoris muscle of Tan sheep were significantly enriched to 327 entries,which were related to fat deposition,such as TCA cycle,respiratory chain complex,hydrogen ion transmembrane transporter activity and so on;28 terms were significantly enriched in longissimus dorsi muscle,including organic acid catabolic metabolism,fatty acid metabolism,extracellular space and so on.KEGG pathway annotation results showed that the differentially expressed genes in biceps femoris muscle of Tan sheep were significantly enriched to 25 pathways,9 of which were involved in fat metabolism,such as TCA cycle,pyruvate metabolism and so on;8 pathways were significantly enriched in longissimus dorsi muscle,2 of which were involved in fat deposition,namely,degradation of valine,leucine and isoleucine and glutathione metabolism.【Conclusion】 By screening the genes related to fat metabolism pathways,MDH2 and PPARGC1A genes in biceps femoris muscle and GSTK1 and MCCC2 genes in longissimus dorsi muscle might be related to fat metabolism in muscles of 9-month-old Tan sheep. The results provided a theoretical reference for further research on fat deposition in muscle tissue of Tan sheep at different growth stages.

Key words: Tan sheep; transcriptome; fatty acid; longissimus dorsi muscle; biceps femoris muscle

中图分类号:

S826

杜佳文, 任文义, 康燕, 徐晓锋, 张力莉. 不同月龄滩羊肌肉组织转录组比较及脂肪酸分析[J]. 中国畜牧兽医, 2023, 50(12): 4925-4934.

DU Jiawen, REN Wenyi, KANG Yan, XU Xiaofeng, ZHANG Lili. Transcriptome Comparison and Fatty Acid Analysis of Muscle Tissue in Tan Sheep at Different Ages[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4925-4934.

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