苏姜猪不同生长阶段背膘组织差异表达基因及其调控通路分析

doi:10.16431/j.cnki.1671-7236.2023.07.001

摘要/Abstract

摘要： 【目的】比较苏姜猪背膘组织生长发育过程中基因表达谱的差异，挖掘影响背膘组织生长发育的关键基因。【方法】选择1、3、6和8月龄（M1、M3、M6、M8）4个生长时期苏姜猪背膘组织进行高通量转录组测序，组成3个比对组（M1 vs M3、M3 vs M6、M6 vs M8）进行差异表达基因（differentially expressed genes，DEGs）的筛选，并进行功能富集和表达趋势分析。【结果】转录组测序显示，M1 vs M3、M3 vs M6、M6 vs M8组分别筛选到1 869、296、1 257个DEGs。功能富集显示，Wnt信号通路、细胞外基质-受体相互作用等信号通路在M1 vs M3组表现活跃，PI3K-Akt信号通路、AMPK信号通路、丁酸代谢、柠檬酸循环等信号通路在M6 vs M8组表现活跃。基因表达趋势分析显示，富含半胱氨酸的酸性分泌蛋白（secreted protein，acid and rich in cysteine，SPARC）、组织蛋白酶S （recombinant cathepsin S，CTSS）、Kruppel样因子7（Kruppel-like factor 7，KLF7）等基因与细胞增殖发育相关，在3月龄时高表达；胰岛素样生长因子-1受体（insulin-like growth factor 1 receptor，IGF1R）、信号转导与转录激活因子1（signal transducer and activator of transcription 1，STAT1）、脂肪酸合成酶（fatty acid synthase，FASN）等基因与细胞脂质代谢相关，在6月龄后表达显著上调。【结论】本研究获得苏姜猪4个生长时期背膘组织的基因表达谱，筛选到SPARC、CTSS、KLF7等与细胞增殖发育相关的关键基因，IGF1R、STAT1、FASN等与细胞脂质代谢相关的关键基因，为苏姜猪背膘厚的遗传改良提供了基础数据。

关键词: 苏姜猪; 转录组测序; 背膘组织; 差异表达基因

Abstract: 【Objective】 The aim of this study was to compare the difference of gene expression profile during the developmental process of the backfat tissue in Sujiang pigs, and explore the key genes affecting the backfat tissue development.【Method】 The backfat tissue in Sujiang pigs at the age of 1, 3, 6 and 8 months (M1, M3, M6 and M8) was selected for high-throughput transcriptome sequencing, and the differentially expressed genes (DEGs) were screened for functional enrichment and expression trend analysis.【Result】 Transcriptome sequencing results showed that 1 869, 296 and 1 257 DEGs were identified in M1 vs M3, M3 vs M6 and M6 vs M8 groups, respectively.Functional enrichment showed that Wnt signaling pathway and ECM-receptor interaction were activated in M1 vs M3 group, while PI3K-Akt signaling pathway, AMPK signaling pathway, butanoate metabolism and citrate cycle (TCA cycle) were activated in M6 vs M8 group.Gene expression trend analysis showed that secreted protein, acid and rich in cysteine (SPARC), recombinant cathepsin S (CTSS), Kruppel-like factor 7 (KLF7) and other genes related to cell proliferation and development were highly expressed at 3 months old, while insulin-like growth factor 1 receptor (IGF1R), signal transducer and activator of transcription 1 (STAT1), fatty acid synthase (FASN) and other genes related to lipid metabolism were significantly expressed after 6 months old.【Conclusion】 This study obtained 4 gene expression profiles of backfat tissue in Sujiang pigs at different growth stages, the key genes related to cell proliferation and development were obtained, including SPARC, CTSS and KLF7, the key genes related to cellular lipid metabolism, including IGF1R, STAT1 and FASN, which provided basic data for genetic improvement of backfat thickness in Sujiang pigs.

Key words: Sujiang pigs; transcriptome sequencing; backfat tissue; differentially expressed genes

中图分类号:

Q786

倪黎纲, 周春宝, 徐盼, 张亚琴, 陶勇, 张君胜. 苏姜猪不同生长阶段背膘组织差异表达基因及其调控通路分析[J]. 中国畜牧兽医, 2023, 50(7): 2595-2605.

NI Ligang, ZHOU Chunbao, XU Pan, ZHANG Yaqin, TAO Yong, ZHANG Junsheng. Analysis of Differentially Expressed Genes and Regulation Pathway of Backfat Tissue in Sujiang Pigs at Different Growth Stages[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2595-2605.

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