›› 2009, Vol. 36 ›› Issue (9): 101-103.

• 遗传繁育 • 上一篇    下一篇

细胞松弛素B对牛GV期卵母细胞玻璃化冷冻效果的影响

郭娟1,2,王志琴1,赵云程2,林嘉鹏1,黄俊成2   

  1. (1.新疆农业大学动物医学学院, 乌鲁木齐 830052; 2.新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室, 乌鲁木齐 830000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-09-20 发布日期:2009-09-20
  • 通讯作者: 黄俊成

Effects of Cytochalasin B on Vitrification of Bovine GV-stage Oocytes

GUO Juan1,2, WANG Zhi-qin1, ZHAO Yun-cheng2, LIN Jia-peng1, HUANG Jun-cheng2   

  1. (1.Department of Veterinary Medicine,Xinjiang Agriculture University, Urumqi 830052,China; 2.The Key Laboratoy of Animal Biotechnology of Ministry of Agriculture, Xinjiang Academy of Animal Sciences,Urumqi 830000,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: HUANG Jun-cheng

摘要: 本试验通过玻璃化冷冻前不同浓度细胞松弛素B(cytochalasin-B,CB)预处理来分析CB对牛GV期卵母细胞固体表面玻璃化(SSV)冷冻后发育潜力的影响。试验结果表明,玻璃化冷冻后,CB处理组和未处理组之间卵母细胞成熟率差异不显著(P>0.05),但均显著低于体外培养组。说明试验中所用CB浓度 (2.5、7.5、15、20和30 μg/mL)对牛GV期卵母细胞玻璃化冷冻效果的改善作用不明显。

关键词: 玻璃化冷冻; 细胞松弛素B; 牛; GV期卵母细胞

Abstract: In the present study, effects of concentration of cytochalasin B(CB) on solid surface vitrification (SSV) of bovine GV-stage oocytes were examined. The results demonstrated that no significant differences were observed in vitro maturation rate among with or without CB pre-treatment,while they were all significantly lower than that in the IVM group.In conclusion,different concentration CB(2.5,7.5,15,20 and 30 μg/mL)treatment before vitrification did not improve the IVM rate following the vitrification of immature bovine oocytes.

Key words: vitrification; cytochalasin B; bovine; GV-stage oocytes

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