›› 2008, Vol. 1 ›› Issue (5): 35-37.

• 生理生化 • 上一篇    下一篇

氟对牛脾淋巴细胞内抗氧化功能和NO代谢的影响

王艳凤, 龚美光, 顾喜娟, 曹言涛, 林洪金   

  1. 东北农业大学动物医学院,哈尔滨 150030
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-05-20 发布日期:2008-05-20

Effects of Fluorine on Antioxdative Activity and Metabolism of Nitric Oxidein the Splenic Lymphocyte of Cattle

WANG Yan-feng, GONG Mei-guang, GU Xi-juan, CAO Yan-tao, LIN Hong-jin
  

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-05-20 Published:2008-05-20

摘要: 采用常规方法分离培养健康牛脾脏淋巴细胞,用不同浓度氟化钠(NaF)染毒24 h。检测牛脾淋巴细胞内谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性及丙二醛(MDA)和一氧化氮(NO)的含量。结果表明,与对照组相比,淋巴细胞内GSH-Px、SOD活性不同程度的降低,而其余各指标均不同程度的升高,且存在着剂量效应关系。由此得出,氟能够导致体外培养牛脾淋巴细胞内抗氧化功能下降,脂质过氧化程度加重,自由基含量增多,对淋巴细胞造成损伤。

关键词: 氟; 牛; 脾脏淋巴细胞; 抗氧化功能

Abstract: Splenic lymphocyte isolated and cultured from cattle spleen was exposed to different of levels of sodium fluoride in 24 h. Detecting GSH-Px activity, SOD activity, NOS avtivity and contents of MDA and NO of splenic lymphocyte. The results showed that GSH-Px activity and SOD activity were higher and other indexes were lower in the lymphocyte exposed to fluorine than control ones and there was doseeffect relationship. Conclusion: it is demonstrated that fluorine could degrade antioxdative activity, aggravate the level of lipid peroxidation, increase the content of free radicals, and induce the damage of lymphocyte.

Key words: fluorine; cattle; splenic lymphocyte; antioxdative activity

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