›› 2007, Vol. 34 ›› Issue (3): 63-66.

• 遗传繁育 • 上一篇    下一篇

松辽黑猪H-FABP基因位点多态性研究

高妍1,肖书奇1,李毅1,赵中利1,袁宝1,张嘉保1,赵志辉2
  

  1. 1.吉林大学实验动物中心,长春 130062; 2.吉林大学畜牧兽医学院,长春 130062
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-03-20 发布日期:2007-03-20

Study on the Polymorphism of Songliao Black Pig Heart Fatty Acid-Binding Protein Gene

GAO Yan1, XIAO Shuqi1, LI Yi1, ZHAO Zhongli1,YUAN Bao1,ZHANG Jiabao1,ZHAO Zhihui2
  

  1. 1.Laboratory Animal Center, Jilin University, Changchun 130062,China; 2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20

摘要: 为检测松辽黑猪H-FABP基因位点多态性,应用PCR-RFLP技术(HinfI、HaeIII和MspI 3种限制性内切酶)检测了62头松辽黑猪5’-上游区和第二内含子的遗传变异情况。结果发现松辽黑猪在MspI多态位点上表现为单一的AA型;而在HinfI多态位点上表现为多态性,等位基因H的基因频率为0.7177,在HaeIII位点上也表现出多态性,等位基因d的基因频率为0.6210。研究结果表明松辽黑猪H-FABP基因5’-上游区和第二内含子存在多态性。

关键词: 松辽黑猪; H-FABP基因; 多态性; PCR-RFLP

Abstract: In order to detect the polymorphism of heart fatty acid-binding protein (HFABP)in the SongLiao black pig , genetic variations of 5’-upstream region and the second intron in 62 SongLiao black pigs H-FABP gene were checked by PCRRFLP with HinfI, HaeIII and MspI.The results indicate that SongLiao black pig only shows sigle AA genotype in MspI-RFLP, for HinfI-RFLP, SongLiao black pig had polymorphism and frequencies of allele H is 0.7177, and for HaeIII-RFLP, SongLiao black pig had polymorphism and frequencies of allele d is 0.6210.So the experiment indicates that SongLiao black pig has polymorphism in the 5’-upstream region and the second intron of H-FABP gene.

Key words: songliao black pig; H-FABP gene; polymorphism; PCR-RFLP

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