动物源性食品和环境中磺胺类药物及其代谢物检测的样品前处理技术研究进展

doi:10.16431/j.cnki.1671-7236.2019.10.025

摘要/Abstract

摘要： 兽用磺胺类药物常添加到动物饲料中用来治疗疾病和促进生长，但不合理的用药会导致磺胺类药物在动物组织中残留，影响食品安全，从而损害人类健康。此外，磺胺类药物在人体及动物体内并不能完全被吸收，大部分以原型及代谢物形式随尿液及粪便排出体外，引起细菌耐药性等一系列问题。因此建立食品和环境中磺胺类药物及其代谢物的检测方法对于保障环境及食品安全，保护人们身体健康具有重要意义。目前，检测磺胺类药物及其代谢物以色谱和质谱等仪器分析方法为主。由于食品和环境中样品基质复杂，药物浓度偏低，所以，在仪器分析前需采取适当的样品前处理对目标物进行富集和纯化。近年来，微型化、无溶剂化、自动化成为样品前处理的发展趋势，作者介绍了固相萃取、固相微萃取、分散液液微萃取、中空纤维液相微萃取、基质固相分散、分子印迹、免疫亲和色谱、场辅助萃取、QuEChERS法、浊点萃取等前处理技术的原理、优缺点及应用，并对未来样品前处理发展方向作出展望，旨在为磺胺类药物及其代谢物的检测提供理论依据。

关键词: 磺胺类药物; 代谢物; 动物食品; 环境; 样品前处理技术

Abstract: Veterinary sulfonamides are often added to animal foods to treat disease and promote growth,however,there is a risk of SAs residues remaining in animal products if these drugs have been administered improperly.Abusing of sulfonamides has generated potentially serious problems in human health and affected food safety.In addition,sulfonamides cannot be completely absorbed in humans and animals and most of them are excreted in urine and feces in the form of prototypes and metabolites,causing a series of problems such as bacterial drug resistance.Therefore,it is necessary to study the residues of sulfonamides in animal products and environment for ensuring food safety and protecting people's health.Currently,chromatography and mass spectrometry are the main methods for the detection of sulfonamides and their metabolites.Pretreatment is necessary in residue detection because of the complex sample matrix and low drug concentrations.In recent years,the sample preparation is developing towards miniaturization,solvent-free and automation.The authors introduced the principles,advantages,disadvantages and application of various sample pretreatment technologies including the solid phase extraction,solid phase microextraction,dispersed liquid phase microextraction,hollow fiber-based liquid-phase microextraction,matrix solid-phase dispersion,molecular imprinting polymers,immune affinity chromatography,field assisted extraction,QuEChERS and cloud point extraction and made outlook pretreatment for the future development direction.

Key words: sulfonamides; metabolites; animal food; environment; pretreatment technique

中图分类号:

S859.84

王玉可, 欧亚红, 张莉蕴, 王延新, 王旭, 彭大鹏, 王玉莲, 潘源虎, 谢书宇, 陈冬梅, 黄玲利, 陶燕飞. 动物源性食品和环境中磺胺类药物及其代谢物检测的样品前处理技术研究进展[J]. 中国畜牧兽医, 2019, 46(10): 3032-3041.

WANG Yuke, OU Yahong, ZHANG Liyun, WANG Yanxin, WANG Xu, PENG Dapeng, WANG Yulian, PAN Yuanhu, XIE Shuyu, CHEN Dongmei, HUANG Lingli, TAO Yanfei. Research Progress on Sample Preparation for Detection of Sulfonamides and Its Metabolites in Animal Food and Environment[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3032-3041.

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