延黄牛ARID5B基因多态性及其与体尺和肉质性状的相关性研究

doi:10.16431/j.cnki.1671-7236.2019.09.016

摘要/Abstract

摘要： 试验旨在研究延黄牛富含AT相互作用功能域5B（AT-rich interactive domain 5B，ARID5B）基因多态性及其与体尺和肉质性状的相关性。选取18月龄的99头延黄牛母牛为研究对象，利用PCR测序查找ARID5B基因13个外显子的SNP位点，通过HRM分型的方法分析SNP位点的多态性，使用SPSS 19.0软件分析该基因多态性与延黄牛体尺和肉质性状的关联性。结果表明，延黄牛ARID5B基因外显子10 Chr28：18186635 bp处存在A/G突变，有3种基因型：AA、AG和GG，其中AA基因型为优势等位基因型，A为优势等位基因；经卡方适合性检验，该SNP位点在延黄牛群体中处于Hardy-Weinberg平衡状态（P>0.05），但A/G突变位点的杂合度相对较低，在延黄牛群体中的变异较小，属于中度多态（0.25 < PIC < 0.5）。关联分析结果表明，ARID5B基因外显子10 GG基因型与延黄牛体尺性状中的体高、胸围和腹围以及肉质性状中的肌内脂肪含量存在显著差异（P<0.05）。本试验揭示了ARID5B基因与牛肉用性状的相关性，为探讨其作为有效遗传标记的可行性提供了参考，为延黄牛分子育种提供试验依据。

关键词: 延黄牛; ARID5B基因; 多态性; 肉用性状; 遗传标记

Abstract: This study was aimed to investigate the polymorphism of AT-rich interactive domain 5B (ARID5B) gene and its correlation with the body size and meat quality traits in Yanhuang cattle.99 Yanhuang cattle at 18 months old were selected as the research object,the SNP of 13 exons of ARID5B gene was found using PCR sequencing,the polymorphism of SNP was analyzed by HRM typing,and the association between ARID5B gene polymorphism and the body size and meat quality traits in Yanhuang cattle was analyzed by SPSS 19.0 software.The results showed that A/G mutation was detected at Chr28:18186635 bp of exon 10 of ARID5B gene in Yanhuang cattle,which contained three genotypes:AA,AG and GG,AA genotype was the dominant genotype,and A was the dominant allele.The chi-square suitability test result showed that Yanhuang cattle population was in Hardy-Weinberg equilibrium (P>0.05),population genetic parameter analysis result showed that the heterozygosity of the mutation site was relatively low,and had a low varitation in Yanhuang cattle population;Polymorphism information content (PIC) analysis showed that it was a moderately polymorphic (0.25 < PIC < 0.5).The correlation analysis results showed that there was a significant difference between the GG genotype of exon 10 of ARID5B gene and the body height,chest circumference,abdominal circumference and intramuscular fat content in Yanhuang cattle (P<0.05).This experiment revealed the correlation between ARID5B gene and meat traits in cattle,which provided a reference for exploring the feasibility as an effective genetic marker,and provided experimental basis for molecular breeding of Yanhuang cattle.

Key words: Yanhuang cattle; ARID5B gene; polymorphisms; meat traits; genetic marker

中图分类号:

S813.3

胡忠昌, 曹阳, 吴健, 秦立红, 金海国, 赵玉民. 延黄牛ARID5B基因多态性及其与体尺和肉质性状的相关性研究[J]. 中国畜牧兽医, 2019, 46(9): 2625-2632.

HU Zhongchang, CAO Yang, WU Jian, QIN Lihong, JIN Haiguo, ZHAO Yumin. Polymorphism of ARID5B Gene and Its Correlation with Body Size and Meat Quality Traits in Yanhuang Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2625-2632.

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