6个猪品种CD53基因中结构变异SV184分布频率的研究

doi:10.16431/j.cnki.1671-7236.2017.04.020

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1079-1086.doi: 10.16431/j.cnki.1671-7236.2017.04.020

6个猪品种CD53基因中结构变异SV184分布频率的研究

徐倩¹, 岑永秀¹, 喻昌燕², 刘畅², 冉雪琴¹, 王嘉福²

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州大学农业工程研究院, 贵阳 550025

修回日期:2017-01-16 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 冉雪琴 E-mail:xqran@gzu.edu.cn;jfwang@gzu.edu.cn
作者简介:徐倩(1990-),女,山东枣庄人,硕士生,研究方向:动物生理与分子生物学,E-mail:qing1317797672@sina.com
基金资助:
国家高技术研究发展计划（863计划）课题（2013AA102503）；贵州省农业攻关项目（黔科合NY[2013]3073号）；贵州省"百"层次创新型人才项目（黔科合人才2016-4012号）；国家自然科学基金（31672390）

Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds

XU Qian¹, CEN Yong-xiu¹, YU Chang-yan², LIU Chang², RAN Xue-qin¹, WANG Jia-fu²

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China

Revised:2017-01-16 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

基于香猪全基因组重测序数据，在白细胞表面抗原CD53基因中筛选到一个结构变异（structure variant，SV）184，为了探索不同猪品种间此结构变异是否存在多态性变化，本试验选择香猪、大白猪、糯谷猪、柯乐猪、江口萝卜猪和荣昌猪作为试验动物，采用PCR方法对猪群中SV184的分布频率进行比较研究，应用RT-PCR技术，分析CD53基因的原初转录本序列结构，研究SV184对CD53基因的转录是否有直接影响。结果显示，6个猪品种中SV184的分布频率存在明显差异，香猪以VN基因型为主，其他5个猪品种是NN基因型占优势；计算两种等位基因的频率，香猪以缺失的V等位基因为主，其他5个猪品种以正常的N等位基因为主。选择3种基因型个体的血液样本，逆转录后分析CD53基因的原初转录本的序列，从中检测到缺失型和不缺失的两种转录本形式，提示SV184对CD53基因的转录无直接影响。SV184可作为分子标记用于区分香猪和其他猪品种。

关键词: 猪品种; CD53基因; 结构变异; 多态性; 转录本前体

Abstract:

A candidate structure variation (SV) 184 was screened out from the resequencing data of Xiang pig. To explore the polymorphism of SV184 among pig breeds, the genotype distributions of SV184 in leukocyte antigen protein (CD53) gene was detected by PCR method taking six Chinese and European pig breeds as samples including Xiang pig,Large White pig,Nuogu pig,Kele pig,Jiangkouluobo pig and Rongchang pig breeds. Furthermore, the primary transcripts of three genotypes of SV184 were determined using RT-PCR technology. Results showed that the type VN was dominant in Xiang pig,while type NN was mainly in others. Both of two primary transcripts of CD53 gene were determined from the heterozygous genotype of SV184,which indicated that the presence of SV184 might not disturb the template selection in the process of CD53 gene transcription. It suggested that SV184 could be taken as one of molecular marker to distinguish Xiang pig from other pig breeds.

Key words: pig breeds; CD53 gene; structure variation; polymorphism; precursors

中图分类号:

S828.8

徐倩, 岑永秀, 喻昌燕, 刘畅, 冉雪琴, 王嘉福. 6个猪品种CD53基因中结构变异SV184分布频率的研究[J]. 《中国畜牧兽医》, 2017, 44(4): 1079-1086.

XU Qian, CEN Yong-xiu, YU Chang-yan, LIU Chang, RAN Xue-qin, WANG Jia-fu. Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds[J]. , 2017, 44(4): 1079-1086.

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6个猪品种CD53基因中结构变异SV184分布频率的研究

Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds

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