拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.01.019

摘要/Abstract

摘要：

试验旨在研究拜城油鸡公鸡中脂肪型脂肪酸结合蛋白（adipocyte fatty acid binding protein，A-FABP）基因的多态性及其与生产性能的相关性，寻找可用于拜城油鸡选育的分子遗传标记。随机选取92只6周龄拜城油鸡公鸡，采用PCR-RFLP方法检测A-FABP基因的多态性。每种基因型选取15只体重相近的个体随机分为3组，每组每种基因型个体各5只，分别在12、18、24周龄时屠宰并对生产性能指标进行测定。结果显示，在拜城油鸡公鸡中检测到A-FABP基因有3种基因型：AA、AB、BB，BB为优势基因型，B为优势等位基因，不符合哈代-温伯格平衡定律（P<0.01）。12周龄时，各基因型间各生产性能指标差异均不显著（P>0.05）；18周龄时，BB基因型肌间脂宽显著高于AA基因型（P<0.05），BB和AB基因型腹脂重极显著高于AA基因型（P<0.01），腹脂率显著高于AA基因型（P<0.05）；24周龄时，BB基因型体重、肌间脂宽、腹脂重均显著高于AA基因型（P<0.05）。推测A-FABP基因可以作为与拜城油鸡公鸡脂肪性状相关的候选标记之一。

关键词: 拜城油鸡; A-FABP基因; 多态性; 生产性能

Abstract:

In order to search the molecular genetic markers for breeding of Baicheng fatty chicken, the relationship between the polymorphism of adipocyte fatty acid binding protein (A-FABP) gene and the productive performance of the male chickens at different ages was studied. Ninety two chickens with age of 6 weeks were randomly selected. The polymorphism of A-FABP gene was detected by PCR-RFLP method. In each genotype group, fifteen chickens with similar body weight were categorized, each group was randomly divided into three sub-groups with equal numbers. The productive performance of the chickens was measured at their age of 12, 18 and 24 weeks. The results showed that there were three genotypes of A-FABP gene:AA,AB and BB, BB genotype was the dominant genotype,allele B was the advantageous allele,and it was not in Hardy-Weinberg equilibrium (P<0.01). At the age of 12 weeks,no significant difference was found among the measured productive performance (P>0.05). At the age of 18 weeks, it was found that the intramuscular fat width of BB genotype was significantly higher than AA genotype (P<0.05), the abdominal fat weight of BB and AB genotypes were extremely significantly higher than AA genotype (P<0.01),and the abdominal fat weight percentage of BB and AB genotypes were significantly higher than AA genotype (P<0.05). At the age of 24 weeks, it was found that the body weight, intramuscular fat width and abdominal fat weight of BB genotype were significantly higher than that of AA genotype (P<0.05). Therefore, this results suggested that A-FABP gene could be used as a potential candidate marker for the association of fatty traits in male Baicheng fatty chicken.

Key words: Baicheng fatty chicken; A-FABP gene; polymorphism; productive performance

中图分类号:

S813.3

邵勇钢, 臧长江, 曾伟丹, 王彦钦. 拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析[J]. 《中国畜牧兽医》, 2017, 44(1): 141-147.

SHAO Yong-gang, ZANG Chang-jiang, ZENG Wei-dan, WANG Yan-qin. Polymorphism of A-FABP Gene and Its Association Analysis with the Productive Performance in Male Baicheng Fatty Chicken[J]. , 2017, 44(1): 141-147.

参考文献

[1] 钟元伦,钟玲,尹家振. 新疆"拜城油鸡"的选育效果[J]. 新疆畜牧业,2008,1:28-30.
[2] 格明古丽·木哈台,王永,等. 拜城油鸡种质特性测定[J]. 中国家禽,2013,35(22):57-60.
[3] Shi H, Wang Q, Wang Y, et al. Adipocyte fatty acid-binding protein:An important gene related to lipid metabolism in chicken adipocytes[J].Comp Biochem Physiol B Biochem Mol Biol, 2010,157(4):357-363.
[4] Wang Q, Guan T, Li H, et al. A novel polymorphism in chicken adipocyte fatty acid-bind protein gene (FABP4) that alters ligand-bind and correlates with fatness[J]. Comp Biochem Physiol B Biochem Mol Biol, 2009, 154(3):298-302.
[5] Wang Q, Li H, Li N, et al. Identification of single nucleotide polymorphism of adipocyte fatty acid-binding protein gene and its association with fatness traits in the chicken[J]. Poult Sci, 2006, 85(3):429-434.
[6] 罗桂芬,陈继兰,文杰,等. 鸡A-FABP基因多态性分析及其与脂肪性状的相关研究[J]. 遗传,2006,28(1):39-42.
[7] 叶满红,文杰,曹红鹤,等. 脂肪型脂肪酸蛋白结合基因多态性与鸡肉品质性状的相关性研究[J].畜牧兽医学报,2007,38(6):526-532.
[8] 王启贵,关天竹,王守志,等. A-FABP基因多态性与肉鸡生长和体组成性状的关联[J]. 遗传,2011,33(2):153-162.
[9] 吴琼,杨福合,邢秀梅,等.中国环颈雉A-FABP基因SNPs及其与屠宰性状和肌内脂肪含量的相关性分析[J].中国畜牧兽医,2012,39(4):126-129.
[10] 吴琼,邢秀梅,徐佳萍,等.中国环颈雉A-FABP基因SNPs检测及其与屠宰性状和肌内脂肪含量的相关性分析[J]. 中国畜牧杂志,2014,50(5):9-13.
[11] 吴琼,荣敏,邢秀梅,等. A-FABP和LPL基因多态性及聚合基因型与中国环颈雉肌内脂肪含量的相关性分析[J].特产研究,2015,1:1-5.
[12] 郭晓敏,王伟,马腾,等. 雪山鸡A-FABP基因编码区SNPs与肌内脂肪酸含量关联分析[J].中国家禽,2014,36(16):17-20.
[13] 王永.和田黑鸡和三黄鸡FABP基因多态性与IMF含量相关性研究[D].北京:中国农业大学,2016.
[14] 常国斌,周琼,栾德琴,等.鸡A-FABP基因不同基因型遗传效应及初步验证[J].畜牧兽医学报,2011,42(8):1088-1094.
[15] Navidshad B, Royan M. Ligands and regulatory modes of peroxisome proliferator-activated receptor gamma (PPARγ) in avians[J]. Crit Rev Eukaryot Gene Expr,2015,25(4):287-292.
[16] Shi H, Zhang Q, Wang Y, et al. Chicken adipocyte fatty acid-binding protein knockdown affects expression of peroxisome proliferator-activated receptor γ gene during oleate-induced adipocyte differentiation[J]. Poult Sci, 2011, 90(5):1037-1044.
[17] 杨宁.家禽生产学[M].第2版. 北京:中国农业出版社,2010.
[18] 陈宽维,章双杰,屠云杰,等. A-FABP在不同鸡种中遗传多态性分析[J]. 畜牧兽医学报,2006,37(11):1114-1117.
[19] 李红伟,陈圆,范红英.惠阳胡须鸡中A-FABP和H-FABP基因单核苷酸多态性的研究[J].惠州学院学报(自然科学版),2016,36(3):22-26.
[20] 刘琛,文杰,赵桂苹,等. A-FABP和AMPD1基因SNP位点对北京油鸡部分肉质性状的组合效应分析[J]. 畜牧兽医学报,2009,40(10):1555-1559.
[21] 高广亮,关天竹,王海威,等. A-FABP基因与肉鸡生长和体组成性状的相关分析[J]. 中国畜牧杂志,2014,50(15):28-32.
[22] 魏伍川,刘欣,陈耀玲. 湛江三黄鸡两个脂肪性状相关基因的多态性及其屠宰腹脂性状的相关分析[J].广东农业科学,2013,2:95-98.
[23] Cahyadi M, Seo D, Choi N, et al. FABP3 and FABP4 genes are the potential candidates for body weights in Korean native chicken[J]. Korean J Poult Sci, 2013,40(2):91-96.