贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测

doi:10.16431/j.cnki.1671-7236.2016.04.037

›› 2016, Vol. 43 ›› Issue (4): 1098-1104.doi: 10.16431/j.cnki.1671-7236.2016.04.037

贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测

曹敏¹, 谭艾娟², 吕世明³, 常鑫¹, 丁昌庆², 陈婷², 王珍燕²

1. 贵州大学药学院, 贵阳 550025;
2. 贵州大学生命科学学院, 贵阳 550025;
3. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2015-10-15 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 吕世明 E-mail:lvlvsm@163.com
作者简介:曹敏(1987-),女,贵州毕节人,硕士生,研究方向:细菌耐药性,E-mail:1195507902@qq.com
基金资助:
贵州省科技厅农业攻关计划项目"生鲜乳安全保障关键技术集成与示范"(黔科合NZ字[2012]3011号);贵州大学研究生创新基金项目"黄酮类化合物对耐药大肠杆菌抗菌增敏作用及机制研究"(研农2015022)

Resistance Analysis and Genotype Detection of ESBLs-producing Swine E.coli Strains in Parts of Guizhou Province

CAO Min¹, TAN Ai-juan², LV Shi-ming³, CHANG Xin¹, DING Chang-qing², CHEN Ting², WANG Zhen-yan²

1. College of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;
2. College of Life Sciences, Guizhou University, Guiyang 550025, China;
3. College of Animal Sciences, Guizhou University, Guiyang 550025, China

Received:2015-10-15 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 为了解贵州地区规模养猪场大肠杆菌菌株耐药情况和ESBLs基因型的流行情况,试验采用CLSI推荐的方法对采自贵州省4个地区规模养猪场的164株大肠杆菌进行药物敏感性试验和产ESBLs菌的检测,并用PCR方法对TEM、SHV、OXA-1和CTX-M-1 4种常见ESBLs基因进行检测。结果显示,164株大肠杆菌对10种常用抗菌药物耐药率分别是头孢噻呋93.29%、氨苄西林87.19%、四环素86.59%、庆大霉素81.10%、链霉素53.66%、多黏菌素51.83%、环丙沙星53.05%、卡那霉素47.56%、金霉素34.76%和氟苯尼考21.95%,且大多为多重耐药,其中检测出ESBLs阳性菌株137株,阳性率为83.54%,各个地区的检出率不同;137株产ESBLs大肠杆菌中,TEM、SHV、OXA-1和CTX-M-1基因的检出率分别为90.51%、70.07%、51.82%和43.07%,且多为复合基因型耐药菌株,各地区的各种基因检出率不同。试验结果表明,贵州部分地区的猪源大肠杆菌耐药现象严重,ESBLs菌株的检出率很高,耐药基因的检出率也极高,且多为复合基因型耐药菌株,应加强当地产酶耐药菌的监测和研究,有效防制此类细菌引发的疾病。

关键词: 猪源大肠杆菌; 耐药性; ESBLs; 基因型

Abstract: To find out the drug resistance and the distribution and prevalence of ESBLs genotypes of E.coli strains on scale pig farms in Guizhou, the antimicrobial susceptibility of 164 E.coli strains were determined.And the genotype of ESBLs was identified and determined by CLSI standard method, DNA was amplified and the 4 genotypes of drug resistance plasmid of ESBLs were analyzed by PCR.The results showed that the drug resistance rates of 164 E.coli strains to ten kinds of antimicrobial agents were ceftiofur 93.29%, ampicillin 87.19%, tetracycline 86.59%, gentamicin 81.10%, streptomycin 53.66%, polymyxin 51.83%, ciprofloxacin 53.05%, kanamycin 47.56%, chlortetracycline 34.76% and florfenicol 21.95%, respectively, and most of them were multiple drug resistance strains, during which there were 137 strains ESBLs positive, detection rate was 83.54%, and the detection rates of 4 regions were different.The detection rates of TEM, CTX-M-1, SHV and OXA-1 genes of 137 ESBLs-producing E.coli strains were 90.51%, 70.07%, 51.82% and 43.07%, respectively.And the detection rates were various in 4 regions.The results showed that the drug resistance of E.coli strains from scale pig farms was serious in Guizhou, and the detection rate of ESBLs strains was very high, the drug resistance gene detection rate was extremely high, and most drug resistance strains were the composite genotype drug resistant.To prevent and cure colibacillosis in veterinary clinic effectively, we should reinforce monitoring and studying about the ESBLs-producing drug resistance bacteria.

Key words: swine E.coli; drug resistance; ESBLs; genotype

中图分类号:

S852.61

曹敏, 谭艾娟, 吕世明, 常鑫, 丁昌庆, 陈婷, 王珍燕. 贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测[J]. , 2016, 43(4): 1098-1104.

CAO Min, TAN Ai-juan, LV Shi-ming, CHANG Xin, DING Chang-qing, CHEN Ting, WANG Zhen-yan. Resistance Analysis and Genotype Detection of ESBLs-producing Swine E.coli Strains in Parts of Guizhou Province[J]. , 2016, 43(4): 1098-1104.

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贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测

Resistance Analysis and Genotype Detection of ESBLs-producing Swine E.coli Strains in Parts of Guizhou Province

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