猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析

doi:10.16431/j.cnki.1671-7236.2022.07.038

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (7): 2796-2804.doi: 10.16431/j.cnki.1671-7236.2022.07.038

猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析

刘宝玲^1,2, 楚品品¹, 李春玲¹, 杨冬霞¹, 蒋智勇¹, 张昆丽¹, 宋帅¹, 勾红潮¹, 蔡汝健¹

1. 广东省农业科学院动物卫生研究所, 广东省畜禽疫病防治研究重点实验室, 农业农村部兽用药物与诊断技术广东科学观测实验站, 广州 510640;
2. 仲恺农业工程学院动物科技学院, 广州 510225

收稿日期:2022-01-07 出版日期:2022-07-05 发布日期:2022-06-29
通讯作者: 蔡汝健 E-mail:466866569@qq.com
作者简介:刘宝玲,E-mail:745292679@qq.com;楚品品,E-mail:cpp1900@163.com。
基金资助:
广州市农村科技特派员项目（20212100015）；2021年英德市科技计划项目；广东省科技计划项目（2016B020202004）；科技创新战略专项资金（高水平农科院建设）（R2019YJ-YB2005、R2020QD-047）

Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines

LIU Baoling^1,2, CHU Pinpin¹, LI Chunling¹, YANG Dongxia¹, JIANG Zhiyong¹, ZHANG Kunli¹, SONG Shuai¹, GOU Hongchao¹, CAI Rujian¹

1. Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province of Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received:2022-01-07 Online:2022-07-05 Published:2022-06-29

摘要/Abstract

摘要： 【目的】了解广东地区猪链球菌（Streptococcus suis，S.suis）临床分离株对四环素类抗生素的耐药性及耐药基因携带情况。【方法】采用药敏纸片扩散法对2016－2020年分离自广东地区的34株猪链球菌进行四环素类抗生素耐药分析，并通过PCR方法检测四环素耐药基因tetA、tetC、tetD、tetM、tetO和tetX携带情况。【结果】34株猪链球菌对四环素的耐药率高达100%（34/34），其次为米诺环素82.4%（28/34）、多西环素47.1%（16/34）。34株猪链球菌中3重耐药菌株有15株，占比44.1%（15/34），2重耐药菌株有14株，占比41.1%（14/34），耐1种药物的菌株5株，占比14.7%（5/34）。耐药基因检测结果显示，tetA、tetC、tetD、tetM、tetO和tetX基因携带率分别为0、0、0、14.7%（5/34）、100%（34/34）和0。【结论】广东地区猪链球菌临床分离株四环素类抗生素的耐药率较高，主要携带的四环素耐药基因为tetO和tetM基因。

关键词: 猪链球菌; 四环素类抗生素; 耐药性; 耐药基因

Abstract: 【Objective】 This study was aimed to learn the resistance of clinical isolates of Streptococcus suis in Guangdong to tetracyclines and the carriage of resistance gene.【Method】 The drug-sensitive disk diffusion method was used to analyze the tetracycline antibiotics resistance of 34 strains of Streptococcus suis isolated from Guangdong during 2016 to 2020.The tetracycline resistance genes tetA,tetC,tetD,tetM,tetO and tetX were detected by PCR.【Result】 The resistance rate of 34 strains of Streptococcus suis to tetracycline was as high as 100% (34/34),followed by minocycline 82.4% (28/34) and doxycycline 47.1% (16/34).Among 34 strains of Streptococcus suis,15 strains were triple-resistant,accounting for 44.1% (15/34),14 strains were double-resistant,accounting for 41.1% (14/34),and 5 strains were resistant to one drug,accounting for 14.7% (5/34).The results of drug resistance gene detection showed that the carrying rates of tetA,tetC,tetD,tetM,tetO and tetX genes were 0,0,0,14.7% (5/34),100% (34/34) and 0,respectively.【Conclusion】 The resistance rate of tetracycline antibiotics in clinical isolates of Streptococcus suis in Guangdong was relatively high,and the main tetracycline resistance genes carried were tetO and tetM genes.

Key words: Streptococcus suis; tetracycline antibiotics; resistance; resistance gene

中图分类号:

S852.61^＋1

刘宝玲, 楚品品, 李春玲, 杨冬霞, 蒋智勇, 张昆丽, 宋帅, 勾红潮, 蔡汝健. 猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析[J]. 中国畜牧兽医, 2022, 49(7): 2796-2804.

LIU Baoling, CHU Pinpin, LI Chunling, YANG Dongxia, JIANG Zhiyong, ZHANG Kunli, SONG Shuai, GOU Hongchao, CAI Rujian. Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2796-2804.

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猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析

Analysis of Resistance and Resistance Genes of Streptococcus suis Clinical Isolates to Tetracyclines

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