金不换水提取物与抗菌药联合对含NDM-1不动杆菌的体外作用研究

doi:10.16431/j.cnki.1671-7236.2016.04.036

›› 2016, Vol. 43 ›› Issue (4): 1092-1097.doi: 10.16431/j.cnki.1671-7236.2016.04.036

金不换水提取物与抗菌药联合对含NDM-1不动杆菌的体外作用研究

吴永继, 刘增援, 庞贞宇, 孙燕杰, 赵雨川, 胡梅, 林立亮, 严泽豪, 黄凯, 司红彬

广西大学动物科学技术学院, 南宁 530005

收稿日期:2015-10-26 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 司红彬 E-mail:zhuli_2009@163.com
作者简介:吴永继(1991-),女,河南驻马店人,硕士生,研究方向:中兽药,E-mail:wyjalisa@163.com
基金资助:
国家自然科学基金(31460675);广西自然基金面上项目(2013GXNSFAA019070);南宁市科技攻关项目(20141295);广西大学大学生创新训练项目(140)

Study on the Effects of Stephania delavayi Diels Water Extracts Combined with Antibiotics on NDM-1-producing Acinetobacter in Vitro

WU Yong-ji, LIU Zeng-yuan, PANG Zhen-yu, SUN Yan-jie, ZHAO Yu-chuan, HU Mei, LIN Li-liang, YAN Ze-hao, HUANG Kai, SI Hong-bin

College of Animal Sciences and Technology, Guangxi University, Nanning 530005, China

Received:2015-10-26 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 本试验旨在探讨金不换水提取物与抗菌药联合对含新德里金属β-内酰胺酶-1 (New Delhi metallo-β-lactamase-1,NDM-1)基因不动杆菌传代的体外抑菌活性,为金不换的综合利用提供参考依据。试验采用二倍微量稀释法体外分别检测金不换水提取物与抗菌药对受试菌的最小抑菌浓度(MIC),并以亚抑菌浓度(即1/2 MIC)的金不换水提取物与抗菌药联合作用对含NDM-1不动杆菌进行传代。结果显示,金不换水提取物的MIC为0.25 g/mL,以0.125 g/mL的金不换水提取物与头孢曲松钠、阿莫西林、头孢噻肟、痢菌净、磺胺间甲氧嘧啶、头孢他啶、头孢噻呋钠、美罗培南8种抗菌药联合作用传代后MIC明显降低。综上所述,金不换水提取物部分联合抗菌药诱导耐药细菌体外抗菌活性明显增强,对含NDM-1的不动杆菌具有一定的抑菌作用。

关键词: 金不换; 含NDM-1不动杆菌; 联合诱导

Abstract: The study was aimed to investigate the antibacterial activity of NDM-1-producing Acinetobacter in vitro induced by water extracts of Stephania delavayi Diels combined with antibacterial agents, and provide reference for the utilization of Stephania delavayi Diels.The minimum inhibitory concentration (MIC) of water extracts of Stephania delavayi Diels and antibacterial agents were tested through twice micro-dilution method.NDM-1-producing Acinetobacter were induced and subcultured by the 1/2 MIC of water extracts of Stephania delavayi Diels combined with antibacterial agents.The results showed that the MIC of water extracts of Stephania delavayi Diels was 0.250 g/mL.The MIC of NDM-1-producing Acinetobacter was decreased obviously after inducing by 0.125 g/mL of water extracts of Stephania delavayi Diels combined with 8 antibacterial agents, such as ceftriaxone sodium, amoxicillin, cefotaxime sodium, mequindox, sulfamonomethoxine, ceftazidime, ceftiofur sodium and meropenem.Antibacterial activities of antibacterial agents against NDM-1-producing Acinetobacter were enhanced obviously by combining with water extracts of Stephania delavayi Diels, and this combination could inhibit NDM-1-producing Acinetobacter to a certain degree.

Key words: Stephania delavayi Diels; NDM-1-producing Acinetobacter; combined induction

中图分类号:

R285

吴永继, 刘增援, 庞贞宇, 孙燕杰, 赵雨川, 胡梅, 林立亮, 严泽豪, 黄凯, 司红彬. 金不换水提取物与抗菌药联合对含NDM-1不动杆菌的体外作用研究[J]. , 2016, 43(4): 1092-1097.

WU Yong-ji, LIU Zeng-yuan, PANG Zhen-yu, SUN Yan-jie, ZHAO Yu-chuan, HU Mei, LIN Li-liang, YAN Ze-hao, HUANG Kai, SI Hong-bin. Study on the Effects of Stephania delavayi Diels Water Extracts Combined with Antibiotics on NDM-1-producing Acinetobacter in Vitro[J]. , 2016, 43(4): 1092-1097.

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金不换水提取物与抗菌药联合对含NDM-1不动杆菌的体外作用研究

Study on the Effects of Stephania delavayi Diels Water Extracts Combined with Antibiotics on NDM-1-producing Acinetobacter in Vitro

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