鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究

doi:10.16431/j.cnki.1671-7236.2015.10.027

›› 2015, Vol. 42 ›› Issue (10): 2695-2700.doi: 10.16431/j.cnki.1671-7236.2015.10.027

鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究

周艳^1,2, 韩海霞^1,2, 逯岩^1,2, 曹顶国^1,2, 李福伟^1,2, 刘玮^1,2, 高金波^1,2, 雷秋霞^1,2

1. 山东省农业科学院家禽研究所, 济南 250023;
2. 山东省家禽育种工程技术研究中心, 济南 250023

收稿日期:2015-02-09 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 雷秋霞 E-mail:jqsyzs@163.com
作者简介:周艳(1979-),女,山东新泰人,博士,副研究员,研究方向:家禽遗传育种,Tel:0531-85990924;E-mail:sally7919@163.com
基金资助:
山东省自然科学基金(ZR2014YL024);山东省自然基金重点项目(ZR2014CZ003);山东省农科院青年基金(2014QNM14);山东省农业良种工程优良家禽新品种培育(2014LZ019)

Polymorphism in PLIN Gene Intron 6 and its Association with Carcass and Fatness Traits in Chicken

ZHOU Yan^1,2, HAN Hai-xia^1,2, LU Yan^1,2, CAO Ding-guo^1,2, LI Fu-wei^1,2, LIU Wei^1,2, GAO Jin-bo^1,2, LEI Qiu-xia^1,2

1. Institute of Poultry Science, Academy of Agricultural Sciences of Shandong Province, Jinan 250023, China;
2. Shandong Poultry Breeding and Engineering Technology Research Center, Jinan 250023, China

Received:2015-02-09 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 试验采用PCR-RFLP方法检测脂滴包被蛋白(perilipin,PLIN)基因内含子6在济宁百日鸡、莱芜黑鸡等4个地方鸡种和1个培育品系中的遗传多态性,分析了多态位点不同基因型与鸡胴体及脂肪性状的相关性。结果发现,在5个供试群体中检测到1个多态位点,测序证实为新发现的鸡PLIN基因2 467 bp处G→A突变,该突变位点在供试群体中检测到3种基因型:A1A1、A1A2和A2A2,2个等位基因:A1和A2。等位基因A1在所有供试群体中均表现为优势等位基因。关联分析结果表明,PLIN基因2 467 bp位点对鸡部分胴体性状和脂肪性状影响显著(P<0.05)。多重比较结果表明,A1A1基因型个体活体重、屠体重、全净膛重、腹脂重和腹脂率均显著高于A2A2基因型个体(P<0.05)。A1A2基因型个体的胸肌肌内脂肪含量显著高于A1A1和A2A2基因型个体(P<0.05)。研究结果表明,PLIN基因对鸡胴体及脂肪性状有一定影响。

关键词: 鸡; PLIN基因; PCR-RFLP; 胴体性状; 脂肪性状

Abstract: The genetic polymorphisms in PLIN gene were detected by PCR-RFLP method in four Chinese local chickens including Jining Bairi chicken,Laiwu Black chicken et al.,and one breeding strain.The correlations between the SNP and the carcass and fatness traits were analyzed.As a result,a novel G→A mutation at 2 467 bp in PLIN gene was identified in the five populations.Three genotypes (A1A1,A1A2 and A2A2) were detected,and allele A1 was predominant in all the five experimental populations.The statistical analysis showed that the 2 467 bp polymorphism locus was significant association with some carcass and fatness traits (P<0.05).The living body weight,carcass weight,eviscerated weight,abdominal fat weight and percentage of abdominal fat of A1A1 genotype was higher than A2A2 genotype in chickens (P<0.05).The breast intramuscular fat of A1A2 genotype was higher than that of A1A1 and A2A2 genotypes in chickens (P<0.05).The results showed that PLIN gene had effect on carcass and fatness traits in chickens.

Key words: chicken; PLIN gene; PCR-RFLP; carcass traits; fatness traits

中图分类号:

S831.2

周艳, 韩海霞, 逯岩, 曹顶国, 李福伟, 刘玮, 高金波, 雷秋霞. 鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究[J]. , 2015, 42(10): 2695-2700.

ZHOU Yan, HAN Hai-xia, LU Yan, CAO Ding-guo, LI Fu-wei, LIU Wei, GAO Jin-bo, LEI Qiu-xia. Polymorphism in PLIN Gene Intron 6 and its Association with Carcass and Fatness Traits in Chicken[J]. , 2015, 42(10): 2695-2700.

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鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究

Polymorphism in PLIN Gene Intron 6 and its Association with Carcass and Fatness Traits in Chicken

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