鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2015.08.034

›› 2015, Vol. 42 ›› Issue (8): 2160-2168.doi: 10.16431/j.cnki.1671-7236.2015.08.034

鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析

施开创¹, 李凤梅², 邹联斌¹, 许心婷², 王海清¹, 钟诚²

1. 广西动物疫病预防控制中心, 南宁 530001;
2. 广西大学动物科学技术学院, 南宁 530005

收稿日期:2015-03-26 出版日期:2015-08-20 发布日期:2015-08-27
通讯作者: 钟诚 E-mail:zhocheng2000@163.com
作者简介:施开创(1968-),男,广西南宁人,博士,研究员,主要从事动物疫病诊断与防控技术研究,E-mail:shikaichuang@126.com
基金资助:
广西水产畜牧科技项目(桂渔牧科1304559)

Isolation,Identification and Analysis of Serotype and Antibiotic Susceptibility of Pathogenic Salmonella from Chickens

SHI Kai-chuang¹, LI Feng-mei², ZOU Lian-bin¹, XU Xin-ting², WANG Hai-qing¹, ZHONG Cheng²

1. Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China;
2. College of Animal Science and Technology, Guangxi University, Nanning 530005, China

Received:2015-03-26 Online:2015-08-20 Published:2015-08-27

摘要/Abstract

摘要： 为了解当前广西鸡源致病性沙门氏菌的优势流行菌株及耐药情况,掌握其最新流行特点,本试验采集疑似病鸡的组织病料,对沙门氏菌进行增菌和分离培养,运用VITEK System ATB Expression法ID 32E肠杆菌鉴定试剂条对分离菌株进行生化试验鉴定,应用玻片凝集试验进行血清型鉴定,采用ATB VET药敏试剂条对30种肠杆菌抗菌药物进行药物敏感性试验。结果显示,从310份疑似鸡病料中分离鉴定出34株致病性沙门氏菌;这些分离菌株中有A群1株、C2群1株、B群15株和D群14株,另有3株未定型,其中以B群鼠伤寒沙门氏菌和D群鸡白痢沙门氏菌为优势菌株;34株分离株均对美洛培南、亚胺培南、大观霉素和安普霉素敏感,对氯霉素、卡那霉素、庆大霉素的耐药率小于10%,对阿莫西林、链霉素、氟甲喹、噁喹酸、磺胺甲噁唑、四环素和呋喃妥因耐药率介于50%和90%之间,而对青霉素、苯唑西林、夫西地酸、利福平和甲硝唑的耐药率达到100%,并且存在多重耐药现象。结果表明,鸡源致病性沙门氏菌的流行菌株具有一定的地域性,当前广西以鼠伤寒沙门氏菌和鸡白痢沙门氏菌为主,流行菌株耐药性严重,应高度关注。

关键词: 沙门氏菌; 鸡; 致病性; 分离鉴定; 血清型; 药物敏感性

Abstract: To investigate the situations of predominant strain and antibiotic resistance of pathogenic Salmonella from chickens in Guangxi Zhuang Autonomous region, Salmonella was pre-enriched and isolated from tissues of clinical suspected chickens, and the Salmonella isolates were identified by biochemical test using ID 32E System for the identification of Enterobacteriaceae of VITEK System ATB Expression, serotypes were determined by slid agglutination test, antibiotic susceptibility was analyzed by ATB VET Susceptibility Test Strip of Enterobacteriaceae antibiotics.The results showed that 34 Salmonella strains were obtained from 310 clinical samples, of which 1 strain belonged to A serogroup, 1 to C2 serogroup, 15 to B serogroup, 14 to D serogroup and 3 to untyped serogroup, and S.typhimurium of B serogroup and S.pullorum of D serogroup were the predominant serotypes.All Salmonella isolates were sensitive to meropenem, imipenem, spectinomycine and apramycine, and the resistance rates to chloramphenicol, kanamycine and gentamicine were less than 10%, and the resistance rates to amoxicillin, streptomycine, flumequine, oxolinique, sulfamethizol, tetracycline and nitrofurantoine ranged between 50% and 90%, while the resistance rates to penicilline, oxacilline, fusidique, rifampicine and metronidazole reached to 100%.The results indicated that the serotype distribution of pathogenic Salmonella from chickens exhibited regional characteristics, and S.typhimurium and S.pullorum were the predominant serotypes in Guangxi Zhuang Autonomous region, and antibiotic resistance of Salmonella isolates was very serious which should be highly concerned.

Key words: Salmonella; chicken; pathogenicity; isolation and identification; serotype; antibiotic susceptibility

中图分类号:

S852.61

施开创, 李凤梅, 邹联斌, 许心婷, 王海清, 钟诚. 鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析[J]. , 2015, 42(8): 2160-2168.

SHI Kai-chuang, LI Feng-mei, ZOU Lian-bin, XU Xin-ting, WANG Hai-qing, ZHONG Cheng. Isolation,Identification and Analysis of Serotype and Antibiotic Susceptibility of Pathogenic Salmonella from Chickens[J]. , 2015, 42(8): 2160-2168.

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鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析

Isolation,Identification and Analysis of Serotype and Antibiotic Susceptibility of Pathogenic Salmonella from Chickens

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