内蒙古地区奶牛源致病性沙门氏菌耐药性分析及ESBLs基因检测

doi:10.16431/j.cnki.1671-7236.2015.08.033

›› 2015, Vol. 42 ›› Issue (8): 2150-2159.doi: 10.16431/j.cnki.1671-7236.2015.08.033

内蒙古地区奶牛源致病性沙门氏菌耐药性分析及ESBLs基因检测

赵俊利^1,2, 石瑞丽³, 李志芳^1,4, 丁月霞^1,4, 李培锋^1,4, 关红^1,4

1. 内蒙古农业大学兽医学院, 呼和浩特 010018;
2. 内蒙古农牧业科学院, 呼和浩特 010031;
3. 内蒙古科技大学包头医学院, 包头 014010;
4. 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018

收稿日期:2015-01-14 出版日期:2015-08-20 发布日期:2015-08-27
通讯作者: 关红 E-mail:guanhong0616@126.com
作者简介:赵俊利(1985-),男,内蒙古呼和浩特人,博士,研究方向:兽医药理学与毒理学,E-mail:Zhaojunli1985@126.com
基金资助:
内蒙古自治区自然科学基金(2012MS0424);中国农业大学与内蒙古农业大学科研合作基金项目(内蒙古地区牛源致病性链球菌耐药性与致病力特征的相关性研究)

Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia

ZHAO Jun-li^1,2, SHI Rui-li³, LI Zhi-fang^1,4, DING Yue-xia^1,4, LI Pei-feng^1,4, GUAN Hong^1,4

1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
3. Baotou Medical College, Inner Mongolia University of Science & Technology, Baotou 014010, China;
4. Key Laboratory of Clinical Diagnosis and Treatment Techniques in Animal Disease, Ministry of Agriculture, Hohhot 010018, China

Received:2015-01-14 Online:2015-08-20 Published:2015-08-27

摘要/Abstract

摘要： 为研究内蒙古地区奶牛源致病性沙门氏菌的耐药特性及ESBLs基因流行特征,试验采用微量肉汤稀释法测定了22种兽医临床常用抗菌药物对临床分离沙门氏菌的最低抑菌浓度(MICs),并对其多重耐药特性进行了分析;采用PCR法对具有β-内酰胺类药物耐药表型的菌株进行了8种动物源沙门氏菌常见ESBLs基因的检测。结果显示,内蒙古地区奶牛源致病性沙门氏菌对甲氧苄啶(97.4%)及磺胺甲基噁唑(94.7%)的耐药率最高,对多数β-内酰胺类、氨基糖甙类、氯霉素类及四环素类药物的耐药性也较为严重(耐药率为40%~80%),所有菌株对氟喹诺酮类药物均敏感;菌株的多重耐药率为94.7%,有29株菌(76.3%)同时对6类抗菌药物具有耐药性;35株对β-内酰胺类药物耐药的菌株中,有30株(85.7%)为ESBLs基因阳性,共检出6种ESBLs基因,其中CTX-M型基因检出率最高(40.0%),未检出SHV和PSE型基因;共发现15种ESBLs基因型,9种ESBLs基因型组合,有16株菌(45.7%)同时携带两种或两种以上ESBLs基因。结果表明,内蒙古地区奶牛源致病性沙门氏菌对兽医临床常用抗菌药物的耐药性较为严重,且ESBLs基因的流行模式较为复杂,提示兽医临床抗菌药物不合理使用可能是造成该地区奶牛源沙门氏菌耐药性产生的原因。

关键词: 奶牛; 沙门氏菌; 耐药性; ESBLs基因

Abstract: The aim of the present study was to investigate the characteristics of antimicrobial resistance and prevalence of ESBLs genes of Salmonella isolates from dairy cows in Inner Mongolia.Micro-dilution broth method recommended by CLSI was used to determine the MICs of 22 antimicrobial agents commonly employed in veterinary clinic against the 38 Salmonella isolates.PCR analysis was carried out to detect the presence of 8 ESBLs genes mostly observed in animal origin Salmonella in the isolates with resistance to at least one β-lactam antibiotics, and the features of multi-resistance of the isolates were also analyzed.The results showed that most Salmonella isolates were resistant to trimethoprim(97.4%), sulfamethoxazole(94.7%), and to most of the β-lactam, aminoglycosides, chloramphenicol and tetracycline antibiotics with the resistance rate ranging from 40% to 80%, however, all of the isolates were sensitive to fluoroquinolone drugs.76.3%(n＝29) of the isolates were simultaneously resistant to 6 categories antibiotics and the multi-resistance rate was 94.7%. 85.7%(n＝30) of the β-lactam antibiotics resistant isolates were ESBLs genes positive.Of the six genotype of ESBLs genes found in this study, CTX-M (40.0%) was the mostly encountered ESBLs gene, however, the SHV and PSE types gene were absent in the isolates.A total of fifteen genotypes of the ESBLs genes and nine ESBLs genes combinations were observed in this study, and 16 isolates (45.7%) simultaneously contained two or more than two ESBLs genes.It was indicated that the current situation of the resistance of the Salmonella isolates from dairy cows in Inner Mongolia to the commonly used antimicrobial agents in veterinary clinic was serious, and the prevalence pattern of the ESBLs genes in the isolates was complex, suggesting that the irrational employment of the antibiotic in the treatment of animal infectious diseases was the crucial reason causing the antimicrobial resistance of the isolates.

Key words: dairy cows; Salmonella; antimicrobial resistance; ESBLs genes

中图分类号:

S852.61

赵俊利, 石瑞丽, 李志芳, 丁月霞, 李培锋, 关红. 内蒙古地区奶牛源致病性沙门氏菌耐药性分析及ESBLs基因检测[J]. , 2015, 42(8): 2150-2159.

ZHAO Jun-li, SHI Rui-li, LI Zhi-fang, DING Yue-xia, LI Pei-feng, GUAN Hong. Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia[J]. , 2015, 42(8): 2150-2159.

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内蒙古地区奶牛源致病性沙门氏菌耐药性分析及ESBLs基因检测

Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia

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