畜禽坏死性肠炎防制研究进展

doi:10.16431/j.cnki.1671-7236.2017.07.032

摘要/Abstract

摘要：

畜禽坏死性肠炎主要是由产气荚膜梭菌引起的一种急性消化道传染病，具有较高的发病率和死亡率。该病不仅损害畜禽健康，影响动物福利，而且对养殖业发展及人类健康造成了严重威胁。抗菌药的出现对该病起到了积极限制的作用，然而随着抗菌药的不合理使用，耐药菌株不断增加，坏死性肠炎的发病率呈现升高的趋势，防制坏死性肠炎面临严峻挑战。作者从坏死性肠炎的特征入手，综述了坏死性肠炎的病原学、理化特性、流行病学、临床症状等，与此同时对坏死性肠炎的防制措施及目前常用药物进行了总结，客观分析了防制坏死性肠炎的趋势与发展方向，旨在健全对该病的认识，为坏死性肠炎的防制研究提供参考。

关键词: 畜禽; 坏死性肠炎; 流行病学; 抗菌药

Abstract:

Necrotic enteritis is an acute gastrointestinal infectious disease with high morbidity and mortality which caused by Clostridium perfringens. The disease not only harms the livestock health and animal welfare,but also has been an emerging threat for breeding industry and human health. Antibacterial drugs played a positive role on preventing this disease,however,drug-resistant strains were increasing with irrational use of antibiotics,the incidence of necrotic enteritis has drastically increased, prevention and treatment of it faced severe challenges. The author reviewed the characteristics of necrotic enteritis include the etiology,physicochemical properties, epidemiology and clinical signs,at the same time, the control measures and common drugs were summarized,and the new development trend and direction for prevention and treatment necrotic enteritis was objective analyzed, aiming to establish a comprehensive understanding of the disease,provide references for prevention and treatment of the disease.

Key words: livestock; necrotic enteritis; epidemiology; antibacterial drugs

中图分类号:

S852.6

王芳, 谢书宇, 潘源虎, 瞿玮, 戴梦红, 黄玲利. 畜禽坏死性肠炎防制研究进展[J]. 《中国畜牧兽医》, 2017, 44(7): 2132-2138.

WANG Fang, XIE Shu-yu, PANG Yuan-hu, QU Wei, DAI Meng-hong, HUANG Ling-li. Research Progress on Prevention and Treatment of Necrotic Enteritis in Livestock[J]. , 2017, 44(7): 2132-2138.

参考文献

[1] Songer J G,Uzal F A. Clostridial enteric infections in pigs[J].Journal of Veterinary Diagnostic Investigation,2005,17(6):528-536.
[2] Lee K W,Lillehoj H S,Jeong W,et al. Avian necrotic enteritis:Experimental models,host immunity, pathogenesis,risk factors,and vaccine development[J].Poultry Science,2011,90(7):1381-1390.
[3] 李云霄,金鑫,张营,等.魏氏梭菌病诊断方法研究进展[J].动物医学进展,2007,28(7):88-93.
[4] Songer J G. Clostridial enteric diseases of domestic animals[J].Clinical Microbiology Reviews,1996, 9(2):216.
[5] 于晓霞.SDS-PAGE等三种方法对产气荚膜梭菌的比较鉴定及类毒素疫苗的初步研究[D].泰安:山东农业大学, 2007.
[6] Freedman J C,Theoret J R,Wisniewski J A,et al. Clostridium perfringens type A-E toxin plasmids[J]. Research in Microbiology,2015,166(4):264-279.
[7] Uzal F A,McClane B A,Cheung J K,et al. Animal models to study the pathogenesis of human and animal Clostridium perfringens infections[J].Veterinary Microbiology,2015,179(1):23-33.
[8] Petit L,Gibert M, Popoff M R.Clostridium perfringens:Toxinotype and genotype[J].Trends in Microbiology,1999,7(3):104-110.
[9] Hafez H M. Enteric diseases of poultry with special attention to Clostridium perfringens[J].Pakistan Veterinary Journal,2011,31:175-184.
[10] Keyburn A L,Bannam T L,Moore R J,et al. NetB,a pore-forming toxin from necrotic enteritis strains of Clostridium perfringens[J].Toxins,2010,2(7):1913-1927.
[11] Dhillon A S,Roy P,Lauerman L,et al. High mortality in egg layers as a result of necrotic enteritis[J].Avian Diseases,2004,48(3):675-680.
[12] Uzal F A,McClane B A. Recent progress in understanding the pathogenesis of Clostridium perfringens type C infections[J]. Veterinary Microbiology,2011,153(1):37-43.
[13] Waters M,Savoie A,Garmory H S,et al. Genotyping and phenotyping of beta2-toxigenic Clostridium perfringens fecal isolates associated with gastrointestinal diseases in piglets[J].Journal of Clinical Microbiology,2003,41(8):3584-3591.
[14] 宣长和.猪病学[M].第2版,北京:中国农业出版社,2003.
[15] Fasina Y O,Newman M M,Stough J M,et al. Effect of Clostridium perfringens infection and antibiotic administration on microbiota in the small intestine of broiler chickens[J]. Poultry Science,2016,95(2):247-260.
[16] Shojadoost B,Vince A R,Prescott J F. The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens:A critical review[J]. Veterinary Research,2012,43:74-86.
[17] 焦静波.猪梭菌性肠炎——仔猪红痢的诊断与综合防治[J]. 畜牧与饲料科学,2009,30(7-8):139-142.
[18] Rahman M M,Kabir M E,Sultana S,et al. Effect of carbohydrate rich diet on experimental pathology of necrotic enteritis in broilers[J].Bangladesh Journal of Veterinary Medicine,2014,12(1):17-26.
[19] Fernando P S,Rose S P Mackenzie A M,et al.Effect of diets containing potato protein or soya bean meal on the incidence of spontaneously-occurring subclinical necrotic enteritis and the physiological response in broiler chickens[J].British Poultry Science,2011,52(1):106-114.
[20] Palliyeguru M W,Rose S P,Mackenzie A M. Effect of trypsin inhibitor activity in soya bean on growth performance,protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks[J].British Poultry Science,2011,52(3):359-367.
[21] Grace N D,Knowles S O. Trace element supplementation of livestock in New Zealand:Meeting the challenges of free-range grazing systems[J].Veterinary Medicine International,2012,2012:1-8.
[22] 中国兽药典委员会,中华人民共和国兽药典兽药使用指南[M].北京:中国农业出版社,2010.
[23] 陈杖榴. 兽医药理学[M].第3版.北京:中国农业出版社,2009.
[24] Benson O. Mineral profile of broilers birds fed zinc bacitracin on different protein diets[J]. Journal of Applied Environmental and Biological Sciences,2013,3(1):7-16.
[25] Sarmah A K,Meyer M T,Boxall A B A. A global perspective on the use,sales exposure pathways,occurrence,fate and effects of veterinary antibioties (VAs) in the environment[J].Chemosphere,2006,65(5):725-759.
[26] Martel A,Devriese L A,Cauwerts K,et al. Susceptibility of Clostridium perfringens strains from broiler chickens to antibiotics and anticoccodials[J].Avian Pathology,2004,33(1):3-7.
[27] Lindstrom M,Heikinheimo A,Lahti P,et al. Novel insights into the epidemiology of Clostridium perfringens type A food poisoning[J].Food Microbiology,2011,28(2):192-198.
[28] Zentek J,Ferrara F,Pieper R,et al. Effects of dietary combinations of organic acids and medium chain fatty acids on the gastrointestinal microbial ecology and bacterial metabolites in the digestive tract of weaning piglets[J].Journal of Animal Science,2013,91(7):3200-3210.
[29] Upadhaya S D,Lee K Y,Kim I H. Influence of protected organic acid blends and diets with different nutrient densities on growth performance,nutrient digestibility and faecal noxious gas emission in growing pigs[J].Vet Med,2014,59:491-497.
[30] Mathis C F,Van Dam J T P.New research into feed additives to control necrotic enteritis[J]. World Poultry,2006,22(3):13-14.
[31] Shalaei M, Hosseini S M,Zergani E. Effect of different supplements on eggshell quality,some characteristics of gastrointestinal tract and performance of laying hens[J]. Vet Res Forum,2014, 5(4):277-286.
[32] Rahimi S,Teymori Zadeh Z,Torshizi K,et al. Effect of the three herbal extracts on growth performance,immune system,blood factors and intestinal selected bacterial population in broiler chickens[J].Journal of Agricultural Science and Technology,2011,13:527-539.
[33] 张建东,许梓荣,詹勇.抗生素类饲料添加剂替代品的研究进展[J].辽宁畜牧兽医,2001,5:30-32.
[34] McReynolds J,Waneck C,Byrd J,et al. Efficacy of multistrain direct-fed microbial and phytogenetic products in reducing necrotic enteritis in commercial broilers[J].Poultry Science,2009,88(10):2075-2080.
[35] 曹力.乳酸杆菌对肉鸡肠道黏膜屏障功能的调节及机理[D].杨凌:西北农林科技大学,2012.
[36] 李鑫,何雅琳.枯草芽孢杆菌PB6对肉鸡产气荚膜梭菌的控制效果研究[J].四川畜牧兽医,2016,1:30-31.
[37] 宫秀燕,刘顺德,韦明,等.凝结芽孢杆菌对感染产气荚膜梭菌肉鸡生长性能、肠道病变及免疫器官指数的影响[J].中国畜牧兽医,2015,42(2):478-486.
[38] Abdel-Raheem S M,Abd-Allah S M S,Hassanein K M A. The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens[J].Int J Agro Vet Med Sci,2012,6:277-289.
[39] Gibson G R,Wang X. Regulatory effects of Bifidobacteria on the growth of other colonic bacteria[J].The Journal of Applied Bacteriology,1994,77(4):412-420.
[40] 曾书琴.抗生素添加剂的负面效应及其替代品的研究动态[J].黑龙江畜牧兽医,2004,2:61-63.
[41] Liu D,Guo S,Guo Y. Xylanase supplementation to a wheat-based diet alleviated the intestinal mucosal barrier impairment of broiler chickens challenged by Clostridium perfringens[J].Avian Pathology, 2012,41(3):291-298.
[42] Hashemi S R,Idrus Z,Bejo M H,et al. Acute toxicity study and phytochemical screening of selected herbal aqueous extract in broiler chickens[J].International Journal of Pharmacology,2008,4(5):352-360.
[43] Awaad M H H,Elmenawey M,Ahmed K A. Effect of a specific combination of carvacrol,cinnamaldehyde, and on the growth performance,carcass quality and gut integrity of broiler chickens[J].Veterinary World,2014,7(5):284-290.
[44] Windisch W M,Schedle K,Plitzner C,et al. Use of phytogenic products as feed additives for swine and poultry[J].Journal Animal Science,2008,86(14):140-148.
[45] Elizondo A M, Mercado E C,Rabinovitz B C,et al. Effect of tannins on the in vitro growth of Clostridium perfringens[J]. Veterinary Microbiology,2010,145(3):308-314.
[46] Lee H S,Ahn Y J. Growth-inhibiting effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria[J].Journal of Agricultural and Food Chemistry,1998,46(1):8-12.
[47] Du E,Gan L,Li Z,et al. In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens[J].Journal of Animal Science and Biotechnology,2015,6(1):1-12.
[48] Karthik K,Muneeswaran N S,Manjunathachar H V,et al. Bacteriophages:Effective alternative to antibiotics[J].Adv Anim Vet Sci,2014,2(3S):1-7.
[49] Soni K A,Nannapaneni R,Hagens S. Reduction of Listeria monocytogenes on the surface of fresh channel catfish fillets by bacteriophage Listex P100[J].Foodborne Pathogens and Disease,2010,7(4):427-434.
[50] Tiwari R,Dhama K,Chakroborty S,et al. Bacteriophage therapy for safeguarding animal and human health:A review[J].Pakistan Journal Biological Science,2014,17(3):301-315.
[51] Abedon S T,Kuhl S J,Blasdel B G,et a1. Phage treatment of human infections[J].Bacteriophage,2011,1(2):66-85.
[52] Loessner M J,Zimmer M,Scherer S. The murein hydrolase of the bacteriophage φ3626 dual lysis system is active against all tested Clostridium perfringens strains[J].Applied and Environmental Microbiology,2002,68(11):5311-5317.
[53] Miller R W,Skinner J,Sulakvelidze A, et al. Bacteriophage therapy for control of necrotic enteritis of broiler chickens experimentally infected with Clostridium perfringens[J].Avian Diseases,2010, 54(1):33-40.
[54] Keyburn A L,Portela R W,Ford M E,et al. Maternal immunization with vaccines containing recombinant NetB toxin partially protects progeny chickens from necrotic enteritis[J].Veterinary Research,2013, 44(1):108.
[55] Shojadoost B,Peighambari S M,Nikpiran H. Isolation,identification,and antimicrobial susceptibility of isolates from acute necrotic enteritis of broiler chickens[J].Int J Vet Res,2010,4(3):147-151.
[56] Ngamwongsatit B,Tanomsridachchai W,Suthienkul O,et al. Multidrug resistance in Clostridium perfringens isolated from diarrheal neonatal piglets in Thailand[J].Anaerobe,2016,38:88-93.