水貂沙门氏菌的分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2019.02.036

Abstract

Abstract:

In order to guide rational drug use and provide the basis for treatment of diseased mink,the bacteria of seven sick minks suffering from salmonellosis provided by mink farms in Shandong province were isolated and identified,and the drug sensitivity was tested.The strains were isolated from the samples by separation and purification methods,and the isolated strains were identified by Gram staining,biochemical identification and PCR.K-B susceptibility test was used to detect the sensitivity of strains to commonly used drugs,and the drug resistance gene and class Ⅰ integrants were detected by PCR.The results showed that 7 strains were isolated,all of which were Gram-negative and short bacilli.Biochemical reaction tests showed that the isolates were positive for glucose,maltose,mannitol,MR test,citrate and hydrogen sulfide test,and the isolates were preliminarily identified as Salmonella.The sequencing results of the PCR products further indicated that 7 isolated strains were Salmonella.The drug sensitivity test showed that 7 strains of Salmonella were sensitive to ceftriaxone,levofloxacin,florfenicol and polymyxin,and resistant to all of the aminoglycosides,tetracycline and ampicillin.The results of drug resistance gene test showed that 8 kinds of resistance genes were detected in 7 strains of Salmonella,bla_TEM-1,bla_CTX-M-1G,aadA1, aac(3')-Ⅳ,aac(3')-Ⅱc,aph(4')-Ⅰa,aph(3')-Ⅶ and oqxAB,and the class Ⅰ integrin of the gene cassettes aaA1,arr-3-aacA4 and bla_PSE-1 were detected.According to the above results,7 strains of Salmonella were isolated from the liver and spleen samples of 7 diseased minks,and the isolates were all resistant strains,and mainly showed multi-drug resistance;The drug resistance genes were diverse,and the presence of drug resistance genes on the plasmid expanded the spread of drug resistance genes and increased the drug resistance of bacteria,which brought difficulties for clinical drug treatment.

Key words: mink; Salmonella; drug sensitivity; drug resistance gene

CLC Number:

S858.92

SUN Na, CHEN Qiang, CHENG Yuening, YANG Yong, WANG Gaili, LIU Zeyu, ZHANG Shuqin, CHENG Shipeng. Isolation,Identification and Drug Sensitivity Analysis of Salmonella from Minks[J]. , 2019, 46(2): 635-641.

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Isolation,Identification and Drug Sensitivity Analysis of Salmonella from Minks

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