饲喂丁酸梭菌对肉鸡盲肠黏膜形态和转录组的影响

doi:10.16431/j.cnki.1671-7236.2023.09.014

Abstract

Abstract: 【Objective】 Feeding with Clostridium butyricum(C. butyricum) in broilers can promote growth and improve feed conversion efficiency,but its mechanism remains unclear.Therefore,the purpose of this study was to investigate the effects of dietary supplementation with C. butyricum on the cecal mucosa in broilers using transcriptome sequencing technology and histological staining,and provide experimental basis and theoretical support for the application of C. butyricum.【Method】 120 Arbor Acres (AA) broilers were randomly divided into 2 groups with 6 replicates each,and each replicate included 10 broilers.The broilers in control group were fed the basal diet,and in test group were fed basal diet supplemented with C. butyricum (1×10⁹ CFU/kg).The trial period was 28 days,with free food and water intake and drinking.1 broiler chicken was randomly selected from each replicate and sacrificed at 28 days of age,and the cecal mucosal sample was taken.RNA-Seq was applied to detect the transcriptomic characteristics of cecum mucosa tissue,and tissue staining was applied to analyze the changes of cecum mucosa.【Results】 123 differentially expressed genes were identified,of which 70 were up-regulated and 53 were down-regulated.Gene Ontology (GO) function enrichment analysis revealed that significant or extremely significant enrichment of functional genes were related to substance transport,cell viability and antioxidant (P＜0.05 or P＜0.01).Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the genes involved in pathway related to vitamin and amino acid metabolism,O-glycan biosynthesis and apelin signaling were the most abundant.Compared with control group,the relative expression of intestinal barrier function related proteins such as Claudin 2,Claudin 15,Claudin 19,Claudin 23,TJP 1,TJP 2,TJP 3 and Mucin 1 in cecal mucosa were significantly or extremely significant increased in test group (P＜0.05 or P＜0.01).HE and PAS staining microscopy of cecal mucosa showed that the depth of intestinal gland,thickness of mucosal layer and thickness of muscle layer,as well as the number and density of goblet cells in test group were significantly higher than those in control group.【Conclusion】 Feeding C.butyricum could improve the substance transport of cecum mucosa,enhance the antioxidant capacity and mucosal barrier function in broiler,thus improving intestinal health and promoting growth performance.

Key words: broiler; Clostridium butyricum; intestinal mucosa; transcriptome sequencing

CLC Number:

S816.7

SUN Xiangli, SHI Ziyao, LI Keke, LIU Yuxuan, WANG Ruolin, LIU Yang, WANG Yanbin. Effect of Feeding Clostridium butyricum on the Morphology and Transcriptomics of Cecum Mucosa in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3584-3593.

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Effect of Feeding Clostridium butyricum on the Morphology and Transcriptomics of Cecum Mucosa in Broilers

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