猪源奇异变形杆菌的分离鉴定及特征分析

doi:10.16431/j.cnki.1671-7236.2021.05.032

Abstract

Abstract: In order to investigate the virulence and drug resistance of Proteus mirabilis from swine in Guangxi province,98 samples of diseased swine were collected from farms with different scales in this study,and identified the isolated bacteria through isolation and culture,morphological observation,straining microscopy,16S rRNA sequencing.Drug susceptibility testing was performed by broth microdilution method.PCR was used to detect virulence genes,drug resistance genes,integrons and variable regions.The variable region amplification products were cloned into pMD^TM19-T for sequencing.The identification results showed that 22 strains of bacteria grew diffusely on the TSA medium,formed a center black marginal white single colony on the SS medium,and microscopic examination showed Gram-negative short bacillus and Proteus specific gene (TUF) positive.The results of 16S rRNA sequencing showed that 21 isolates had 99% homology with Proteus mirabilis,and one isolate had 99% homology with Proteus penneri.The drug sensitivity results showed that the drug resistance rate of 21 strains of Proteus mirabilis to tetracycline,doxycycline,ampicillin and sulfamethoxazole reached 100%,the drug resistance rate of cefalexin,cefuroxime,cefotaxime,imipenem and kanamycin was above 57.1%,and all the isolated strains were multiple drug resistant bacteria.PCR results showed that the virulence gene detection rates of atfA,hpmA,ireA,mrpA,pmfA,rsbA,ureC,zapA and ptA were all 100% and that of ucaA was 95.2%.The strains of ESBLs were bla_TEM type,bla_CTX type or bla_TEMtype and bla_CTX type,and the AmpC strains were bla_DHAtype.The proportion of strains carrying ESBLs or AmpC genes was 57.1%, 14.3%, and the proportion of strains carrying both ESBLs and AmpC genes was 14.3%.The detection rates of qnrA,qnrB,qnrS and aac(6')-Ⅰb-cr were 9.5%,0,4.8% and 80.1%,respectively.21 strains of isolated bacteria classⅠ integron (intⅠ1) positive rate was 61.9%,nine different gene cassette arrays were detected (aadA2-linF,estX,dfrA32-ereA-aadA2,drfA5,drfA12-orfF-aadA2,arr3-aac(6')-Ⅰb-cr5,aac(6')-Ⅰb-cr5-bla_OXA-1-catB3-aar3,drfA1-orfC and aadA2);classⅡ integron (intⅠ2) positive rate was 76.2%,only one gene cassette array (drfA1-sat1-aadA1) was detected.The results of this study showed that the virulence and drug resistance of swine-origin Proteus mirabilis were high in Guangxi,which provided data support for the prevention and control of Proteus mirabilis.

Key words: Proteus mirabilis; drug sensitivity test; virulence genes; antibiotic resistance genes; integron

CLC Number:

S852.61

GE Qiang, MA Dongxin, ZHOU Yuqing, CHEN Huaijun, YUAN Jingzhi, LI Xun, WANG Xiaoye. Isolation,Identification and Biological Characteristics of Proteus mirabilis of Swine[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1804-1815.

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Isolation,Identification and Biological Characteristics of Proteus mirabilis of Swine

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