盘羊及其杂交后代微卫星遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2020.04.016

Abstract

Abstract: In order to better understand the genetic information of Argali sheep and its hybrid offspring (Argali sheep ♂×Oula sheep ♀),and provide a theoretical basis for the evaluation genetic resources of Argali sheep and the rejuvenation and improvement of Oula sheep,24 microsatellite loci were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 24 Argali sheep and 24 hybrid sheep.The results showed that,167 alleles were detected at 24 microsatellite loci in 24 Argali sheep,the average number of alleles was 6.9583,the average effective allele was 3.3665,the average observed heterozygosity was 0.4497,the average expected heterozygosity was 0.6779,and the average polymorphic information content was 0.6265,MAF70 locus had the highest numbers of alleles,OarFCB193 locus had the highest numbers of effective alleles,expected heterozygosity and polymorphism information content,ILSTS11 locus had the highest observe heterozygosity.In 24 hybrid sheep,154 alleles were found in 24 microsatellite loci,the average number of alleles was 6.4166,the average effective allele was 3.4061,the average observed heterozygosity was 0.4566,the average expected heterozygosity was 0.6751,and the average polymorphic information content was 0.6265.MAF70 locus had the highest numbers of alleles,numbers of effective alleles,expected heterozygosity and polymorphism information content,OarJMP58 locus had the highest observe heterozygosity.It was found that the genetic indicators of Argali sheep and hybrid offspring were similar in genetics,and the data could be used for evaluate the genetic resources of Argali sheep and the rejuvenation and improvement of Oula sheep.

Key words: Argali sheep; Oula sheep; microsatellite; genetic marker

CLC Number:

S813.3

CHI Haobin, LANG Xia, WANG Cailian, LIU Lishan. Analysis of Microsatellite Genetic Diversity in Argali and Its Hybrid Offspring[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1109-1120.

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Analysis of Microsatellite Genetic Diversity in Argali and Its Hybrid Offspring

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