China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (4): 76-79.

• 生物技术 • Previous Articles     Next Articles

Research and Assessment of Real-time Quantitative PCR Assay for the Detection of African Swine Fever Virus

GUO Shao-ping1，2，LIU Jian2，WU Shao-qiang2   

  1. (1.Inner Mongolia Agricultural University, Hohhot 010018, China;2.Chinese Academy of Inspection and Quarantine, Beijing 100029, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: WU Shao-qiang

Abstract: A real-time quantitative PCR（qPCR）assay for the detection of African swine fever virus（ASFV）has been developed by designing the primers and TaqMan probe according to the sequence of the gene K205R. The reaction solutions and conditions were optimized by using different concentrations of the primers and Mg 2+ and different annealing temperature. The mask samples were adopted to verify its actual detective efficiency by adding the plasmid DNA into swine liver tissue. The qPCR detective method of ASFV based on p72 recommended by OIE was selected and modified to be as control method. Results showed that the amplification efficiency of the developed qPCR based on detection of K205R was better than that based on p72 and was sensitive and specific enough to detect African swine fever virus infection.

Key words: African swine fever virus; real-time quantitative PCR; K205R; detection; research; assessment

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