冷应激后民猪肝素结合性表皮生长因子基因表达研究

Abstract

Abstract: Hepafin-binding epidermal growth factor-like (HB-EGF) is one of the epidermal growth facto family. In this study, Real-time PCR method was used to detect and analysis the expression of HB-EGF gene in Min pig skeletal muscle after cold-induced. The results showed that the low-temperature group(－20 ℃) of HB-EGF gene expression levels compared with the normothermia group (10 ℃) appeared very significantly decreased (P＜0.01). As above, cold stress inhibits the expression of HB-EGF gene, but also indirectly inhibit the normal growth of Min pig.

Key words: Min pig; cold stress; HB-EGF; Real-time PCR

CLC Number:

Q786

ZHANG Dong-jie;LIU Di;WANG Liang;BIE Shu;SUN Hong-tao;YANG Guo-wei. Study on Expression of Min Pig Hepafin-binding Epidermal Growth Factor-like Gene in Cold Stress[J]. , 2011, 38(4): 98-100.

[1]	SHI Hongzhao, LIU Yang, YAO Ruizhi, LIU Peng, LI Shize. Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 932-941.
[2]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[3]	SUN Changchao, GAO Xiang, LI Feng, LIANG Tong, NING Hangyi, WANG Zhongyu, SHAN Anshan, SHI Baoming. Effects of Short-term Administration of Bacillus velezensis M2 Isolated from Min Pigs on the Growth,Immunity and Intestinal Microflora of SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1264-1274.
[4]	LIU Yanguang, LUO Xinhui, LIU Jianqiang, JIA Qi, XIAO Cheng, CAO Yang, JIN Haiguo, ZHANG Lichun. Analysis of Polymorphism and Expression Profile of PBD-110 Gene in Pigs with Different Genetic Background [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 975-981.
[5]	WANG Yanan, FENG Man, ZHOU Yinghao, MAO Sen, QIU Dianrui, GUO Jianjun, LI Suxia, WANG Hongbin. Study on the Change Law of Milk Production Traits and Routine Blood Indicators of Holstein Dairy Cows in the North of Hebei Province in Autumn and Winter [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1282-1288.
[6]	ZHANG Dongjie, WANG Liang, LIU Yang, LIU Di. Screening and Analysis of Transcription Factors Regulating Tβ4 Gene in Min Pig [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2535-2542.
[7]	WEI Dianhua, GAO Jingwen, WANG Xiaoxuan, ZHANG Li, QI Yayin. Effects of Acute Cold Stress on mRNA Expression of HSP60,HSP70 and HSP90 Genes in Altay Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3680-3689.
[8]	JIANG Wenjie, LI Yinghua, YAO Xuerui, ZHAO Yuhan, GAO Qingshan, PIAO Chunhao, SUN Fuliang, XU Yongnan. Effects of Cold or Heat Stress on the Development of Porcine Parthenogenetic Embryos in vitro [J]. , 2018, 45(9): 2507-2515.
[9]	ZHANG Li-chun, LI Zhao-hua, LI Meng-shu, SUN Fu-liang, CAO Yang, PIAO Qing-lin, JIN Hai-guo, ZHANG Shu-min. The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background [J]. , 2017, 44(4): 1046-1053.
[10]	MA Hong, LIU Yu, WANG Liang, WANG Wen-tao, WU Sai-hui, HE Xin-miao, LIU Di. Screening of the Reference Genes for the Study of Peripheral Blood Mononuclear Cells by Quantitative Real-time PCR in Min Pigs [J]. , 2017, 44(12): 3426-3433.
[11]	LI Ling, ZHANG Dong-jie, WANG Liang, WANG Xin-xin, CAO Yue, LIU Di. Cloning and Bioinformatics Analysis of FoxN1 Gene of Min Pig [J]. , 2016, 43(4): 928-933.
[12]	YANG Li, ZHANG Li, QI Ya-yin, SHEN Wen, LI Yan, ZHANG Lu-an. Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep [J]. , 2015, 42(4): 890-895.
[13]	ZHANG Ying-chao, WANG Xi-biao, CUI Shi-quan, DI Sheng-wei, GUAN Sen. Study on Relationship between Intestinal Epithelium Tight Junction Proteins mRNA Expression and Piglets Weaning Diarrhea [J]. , 2014, 41(9): 221-224.
[14]	YANG Li, QI Ya-yin, ZHANG Li, SHEN Wen, LI Yan, ZHANG Lu-an. Effect of Cold Stress on the Lipoprotein Lipase Gene mRNA Expression in Altay Sheep [J]. , 2014, 41(12): 230-234.
[15]	XU Zong-li, XIE Zhi-xun, XIE Li-ji, LIU Jia-bo, XIE Zhi-qin, PANG Yao-shan, DENG Xian-wen, FAN Qing. Establishment of a Duplex Real-time PCR Assay for Detection of Duck Paramyxovirus and Duck Circovirus [J]. , 2013, 40(4): 26-31.

Study on Expression of Min Pig Hepafin-binding Epidermal Growth Factor-like Gene in Cold Stress

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments