冷、热应激对猪孤雌胚胎体外发育的影响

doi:10.16431/j.cnki.1671-7236.2018.09.021

Abstract

Abstract:

In order to study the effects of cold and heat stress at different time on the development of porcine parthenogenetic embryos in vitro,the porcine parthenogenetic embryos were used as the material in this study,immunofluorescence staining and Real-time PCR were adopted to detect the rate of blastocyst,cell count,cell apoptosis,and expression of autophagy related gene and transcription level of apoptosis related gene mRNA.The results showed that compared with control group,the rate of blastocyst decreased significantly after 12 h heat stress or cold stress for 18 h (P<0.05),while the rate of blastocyst in cold stress group was higher than that in heat stress group.After 12 h heat stress or cold stress for 18 h,the number of cells in blastocyst decreased significantly (P<0.05),the apoptotic rate increased significantly (P<0.05),and the apoptotic rate of cold stress group was lower than that in heat stress group.The expression of autophagy related genes Atg6 and Atg8 in cold and heat stress groups was extremely significantly higher than that in control group (P<0.01),Lamp2 expression was significantly higher than that in control group (P<0.05),and Atg6 and Atg8 genes expression in heat stress group were higher than that of cold stress group.By detecting the transcription level of apoptosis-related gene mRNA,the expression of apoptosis-related genes Bak,Casp-3 and FAs in cold and heat stress group was extremely significantly higher than that in control group (P<0.01),and Bcl-xl expression was significantly lower than that in control group (P<0.05).In summary,the tolerance of porcine parthenogenetic embryos to cold stress (31℃) was stronger than that of heat stress (41℃),and heat stress could induce the expression of autophagy and apoptosis-related genes in porcine parthenogenetic embryos cultured in vitro,that could reduce the development ability of parthenogenetic embryos.

Key words: cold stress; hot stress; porcine parthenogenetic embryos; autophagy; apoptosis

CLC Number:

S828

JIANG Wenjie, LI Yinghua, YAO Xuerui, ZHAO Yuhan, GAO Qingshan, PIAO Chunhao, SUN Fuliang, XU Yongnan. Effects of Cold or Heat Stress on the Development of Porcine Parthenogenetic Embryos in vitro[J]. , 2018, 45(9): 2507-2515.

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Effects of Cold or Heat Stress on the Development of Porcine Parthenogenetic Embryos in vitro

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