不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析

doi:10.16431/j.cnki.1671-7236.2017.04.016

Abstract

Abstract:

In order to explore the polymorphisms of myxovirus resistance (Mx1) gene in pig populations from different genetic background,we cloned Mx1 gene and tested the polymorphisms on exon 2 of Mx1 gene in Min pig, crossbred from wild boars in Changbai Mountain with Min pig (crossbred wild boars) and Large White pig population using high resolution melting (HRM). The results showed that four Mx1 mRNAs were cloned from Min pig and crossbred wild boars, and they had whole ORF which was 1 992 bp in length and coded 663 amino acids. In the four Mx1 mRNA, there were 30 SNPs in nucleotide and 17 mutations in amino acid. The location of SNPs displayed that almost mutations located on two sides of the function domains in Mx1 protein. The polymorphism data in HRM test showed that the DD genotype was the dominant genotype which had the highest frequency in three populations. The DD genotype frequency was 100% in Large White pig population, 62.5% in crossbred wild boars, and 56.2% in Min pig.Some novel mutations in both nucleotide and amino acid on exon 2 region were explored in polymorphism test. In conclusion, there was more polymorphism of Mx1 gene in Min pig and crossbred wild boars.

Key words: Mx1 gene; Min pig; Large White pig; crossbred wild boars; gene polymorphism

CLC Number:

S813.3

ZHANG Li-chun, LI Zhao-hua, LI Meng-shu, SUN Fu-liang, CAO Yang, PIAO Qing-lin, JIN Hai-guo, ZHANG Shu-min. The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background[J]. , 2017, 44(4): 1046-1053.

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The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background

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