Abstract: The experiments were designed to study the changed of T lymphocyte subset about CD3^＋,CD4^＋,CD8^＋ which detected by triple-color flow cytometry for swine,the blood sample were collected by three different anticoagulants and detection on different days. The results showed that CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd,4th day had no significant difference,but on the 6th CD4^＋,CD8^＋ had significant different,on the 8th day and 10th day had extremely significant different in the heparin anticoagulant samples group. In the EDTA anticoagulant samples group CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd and 4th day had no significant difference,on the 6th day CD3^＋ had significant difference,on the 8th and 10th day had extremely significant difference. In the sodium citrate anticoagulant samples group CD3^＋,CD4^＋,CD8^＋ detected ratio on the 1st,2nd,3rd,4th,6th day and the 8th day had no significant difference,on the 10th day CD3^＋,CD4^＋ had extremely significant difference,and CD8^＋ had significant difference.Compared three different anticoagulants,the heparin was better others,and it’s better to detect the samples within four days.

Key words: flow cytometry; T lymphocyte subset; anticoagulants; sample collection