›› 2011, Vol. 38 ›› Issue (4): 101-104.

• 生物技术 • Previous Articles     Next Articles

The Effects of T Lymphocyte Subset Detection for Flow Cytometry under Different Experimental Conditions

QU Xue-qi1, ZHAO Jian-zeng1,2, LIANG Zhi-xuan3, LI Yong1, BAI Xue1, MEI Lin1, WEN Tao2, HE Bin1, YANG Ju1, LUO Sheng-jun4, XING Hai-yun2, GAO Ying-jie1   

  1. 1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Beijing Bigvet Biotech Co.Ltd.,Beijing 100176,China;3. Tianjin Center for Disease Control and Prevention,Tianjin 300402,China;4. Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
  • Received:2010-10-26 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

Abstract: The experiments were designed to study the changed of T lymphocyte subset about CD3＋,CD4＋,CD8＋ which detected by triple-color flow cytometry for swine,the blood sample were collected by three different anticoagulants and detection on different days. The results showed that CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd,4th day had no significant difference,but on the 6th CD4＋,CD8＋ had significant different,on the 8th day and 10th day had extremely significant different in the heparin anticoagulant samples group. In the EDTA anticoagulant samples group CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd and 4th day had no significant difference,on the 6th day CD3＋ had significant difference,on the 8th and 10th day had extremely significant difference. In the sodium citrate anticoagulant samples group CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd,3rd,4th,6th day and the 8th day had no significant difference,on the 10th day CD3＋,CD4＋ had extremely significant difference,and CD8＋ had significant difference.Compared three different anticoagulants,the heparin was better others,and it’s better to detect the samples within four days.

Key words: flow cytometry; T lymphocyte subset; anticoagulants; sample collection

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