单细胞转录组测序技术在精子发生研究中的应用进展

doi:10.16431/j.cnki.1671-7236.2024.10.021

Abstract

Abstract: Spermatogenesis is a highly heterogeneous process in time and space that occurs in the mammalian testis through a series of cellular differentiation and development，culminating in the formation of spermatozoa. With the continuous updating and development of bioinformatics，most previous studies have relied on the analysis of RNA from various cell types. However，isolating each cell type from the testis is very challenging，and therefore relatively few studies have been conducted on different types of testicular germ cells at the transcriptome level. In recent years，due to the progress and development of science and technology，single-cell RNA sequencing (scRNA-Seq) has introduced a variety of sequencing platforms，and its scope of use and scenarios continue to expand，with a wide range of applications in the fields of neurology，oncology，immunity，growth and development and disease，and the research objects are mainly related to human，model organisms and domestic animals. This technology can efficiently capture individual cells in the testis for high-throughput sequencing analysis to obtain their transcriptome-level information，finely characterize their cell types，reveal their cellular heterogeneity，and infer their developmental trajectories. Currently，scRNA-Seq has begun to explore the mechanisms related to spermatogenesis in livestock from the perspective of livestock germ cells，so as to further study the processes related to spermatogenesis in livestock. Currently，scRNA-Seq has begun to explore the mechanisms of spermatogenesis in livestock from the perspective of livestock germ cells，so as to further study the process of spermatogenesis in livestock. Therefore，based on the description of scRNA-Seq technology，the author summarizes and discusses its application and research progress in the field of spermatogenesis in domestic animals such as cattle，sheep and swine，so as to provide a theoretical basis for the study of spermatogenesis in domestic animals.

Key words: single-cell transcriptome sequencing; spermatogenesis; domestic animal

CLC Number:

S814.8

MA Yuan, JIN Haoyan, WANG Nana, LI Qihan, ZHANG Lingkai. Progress in the Application of Single-cell Transcriptome Sequencing Technology in Spermatogenesis[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4400-4409.

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Progress in the Application of Single-cell Transcriptome Sequencing Technology in Spermatogenesis

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