不同产驹数阳原驴血液转录组分析

doi:10.16431/j.cnki.1671-7236.2024.10.020

Abstract

Abstract: 【Objective】 The purpose of this experiment was to screen the key genes and related pathways that affected the litter size of Yangyuan donkeys，and improve the reproductive performance of Yangyuan donkeys. 【Method】 The venous blood samples of healthy Yangyuan donkeys with twins and singleton were used for transcriptome high-throughput sequencing. After comparison with the reference genome of the African wild donkey (Equus asinus (ass))，the differentially expressed genes were screened,GO function and KEGG pathway enrichment analysis were performed on the differentially expressed genes to screen the genes related to the litter size of Yangyuan donkeys. Five differentially expressed genes were randomly selected,and Real-time quantitative PCR was used for expression quantitative verification. 【Result】 A total of 1 336 differentially expressed genes were screened out between twins and singleton groups，among which 723 genes were upregulated，and 613 genes were downregulated. GO function enrichment analysis showed that the differentially expressed genes were mainly enriched in receptor activity，signal transduction and other terms. KEGG pathway enrichment analysis showed that ovarian steroidogenesis and estrogen signaling pathways were in top 20 enriched pathways，and a total of 4 candidate genes related to the litter size of Yangyuan donkeys were screened out，including IGF1，IGF1R，INSR and ADCY3. Real-time quantitative PCR results were consistent with the transcriptome sequencing results，and the transcriptome sequencing results were reliable. 【Conclusion】 The enrichment of differentially expressed genes in Yangyuan donkeys with different number of foals was found in ovarian steroidogenesis and estrogen signaling pathways，IGF1，IGF1R，INSR and ADCY3 genes were involved in ovarian hormone secretion and up-regulated in twins group. The results provided a reference for further clarifying the molecular mechanism of different litter sizes in Yangyuan donkeys.

Key words: Yangyuan donkeys; litter size; transcriptome; reproductive performance

CLC Number:

S822

WANG Maohan, SU Yongmei, ZHU Wenjin, CHEN Liang, NI Huiyong, LU Chunxiang, WANG Maosen, SHI Feng, ZHAO Chenkun. Transcriptome Analysis of Blood in Yangyuan Donkeys with Different Litter Size[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4391-4399.

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Transcriptome Analysis of Blood in Yangyuan Donkeys with Different Litter Size

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