水牛信号转导子和转录激活子1基因电子克隆及序列分析

Abstract

Abstract: The buffalo STAT1 gene was silico cloned and analyzed by bioinformatics method in this study. Using the Bos taurus STAT1 sequences to get and identify the buffalo STAT1 gene sequences by the silico cloning and RT-PCR technology. The results showed that the buffalo STAT1 gene length size was 3437 bp, contained an open reading frame (ORF) of 2244 bp that encoded 747 amino acids. The CDS of the obtained buffalo STAT1 gene showed that 99%，98%，89%，93%，86%，85% and 87% identity with that of Bos taurus, Ovi saries, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus and Capra hircus. Analysis of the phylogenetic tree showed that the nearest relationship existed between buffalo and Bos taurus.

Key words: buffalo; STAT1 gene; silico cloning; bioinformatics

DENG Ting-xian, PANG Chun-ying, CHEN Ming-tang, WANG Jian, YANG Bing-zhuang, LIANG Xian-wei. Silico Cloning and Sequence Analysis of STAT1 Gene in Water Buffalo (Bubalus bubalis)[J]. .

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Silico Cloning and Sequence Analysis of STAT1 Gene in Water Buffalo (Bubalus bubalis)

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