Abstract: To establish a rapid duck enteritis virus (DEV) pathogen detection method, according to the DEV gene sequence in GenBank, we synthesized outer and inner two pairs of primers, established a nested PCR for DEV detection. The method used for normal duck embryo, healthy duck constitution, infectious laryngotracheitis virus (ILTV), pseudorabies virus (PRV), egg drop syndrome virus (EDSV), duck hepatitis virus (DHV) and Newcastle disease virus (NDV), all of the PCR results were negative, the sensitivity of first amplification was 10 ng, the second amplification sensitivity was 0.1 ng, the second amplification was more sensitive 100 times than the first amplification. The established nest PCR method had good specificity, sensitivity, it could detect low levels of DEV quickly and accurately, it provided a kind of efficient, rapid, specific and sensitive detection method for pathogen detection and molecular epidemiology of material such as of duck viral enteritis.

Key words: Anatid herpesvirus 1; nest PCR; detection