Abstract: In order to study the effect of myostatin (MSTN) gene on the meat performance of Local Yellow cattle in China,selecting 2 Local cattle breeds (Grassland Red bull,Yanbian cattle) as the research object,four binary hybrid cattle group (Germany Yellow cattle×Simmental,Limousin×Simmental,Charolais×Simmental and Red Angus×Simmental) as reference.PCR-RFLP and DNA sequencing methods were conducted to detect single nucleotide polymorphism of MSTN gene in two Chinese native cattle and four cross cattle,and analyzed the correlations between single nucleotide polymorphism of China Red Steppe MSTN gene and slaughter traits.The sequence analysis showed that there was no mutations in the MSTN gene exon 3,a C5358A mutation of 3'-UTR of MSTN gene was found,which made the AclⅠrestriction enzyme site disappeared,all cattle groups showed polymorphism which was formed two genes as A,C, and 3 genotypes as AA,CA and CC, respectively.The research results suggested that AA genotype in groups of four hybrid cattle had dominant advantage and the genotype frequency was apparently higher than two groups of Chinese Native cattle for the test of independence,allele A was expressed the preferential allelic,the frequencies of CA,CC and AA genotypes showed a little variation between two cross cattle and Native cattle,and the frequency of allele C was apparently higher than allele A,so allele C was designed the preferential allelic in the local population.While correlations between different genotypes of China Red Steppe and slaughter traits analysis showed that AA genotype individual net meat percentage and eye muscle area were significantly higher than CA and CC genotype individuals (P<0.05),but other slaughter straits had no significant differences in three genotypes(P>0.05).These results provided a useful reference for research of the meat performance of China Red Steppe in the molecular breeding field.

Key words: China Red Steppe; MSTN gene; correlation analysis