Abstract: To establish a rapid rabbit hemorrhagic disease virus (RHDV) pathogen detection methods, this research based on RHDV gene sequence in GenBank, synthesized outer and inner two pairs of primers, and established a nested RT-PCR for RHDV detection. The method used for Lapine rotavirus (LaRV), Sendai virus(SeV) and healthy rabbit constitution, all of the RT-PCR results were negative; the method for first amplification the sensitivity was 10 ng, the second amplification sensitivity was 0.1 ng, the second amplification was 100 more times sensitive than the first amplification. The established nest RT-PCR method had good specificity and sensitivity, it could detect very low levels of RHDV quickly and accurately, it provided a kind of efficient, rapid, specific and sensitive detection method for pathogen detection and molecular epidemiology of material such as of RHD.

Key words: rabbit hemorrhagic disease virus; nest RT-PCR; detection