牛病毒性腹泻病毒JN株的分离鉴定及E2基因的序列分析

Abstract

Abstract: A strain of bovine viral diarrhea virus (BVDV) was isolated and identified from the suspected of bovine viral diarrhea virus infection cattle secretions and excretions, and E2 gene sequence was analysed. The results showed that the isolated virus was named as JN strain, Reed-Muench method for the determination of isolate virus TCID₅₀ was 10^-7.5/0.1 mL, virus neutralization test results showed that the BVDV JN isolate could be specifically neutralized by BVDV positive serum, but not by BVDV negative serum neutralization. Results of E2 gene sequences of BVDV isolate showed that the isolate belonged to the BVDV Ⅰa subtype.

Key words: bovine viral diarrhea virus; isolation and identification; E2 gene; sequence analysis

CLC Number:

S852.65⁺3

TANG Zhong-he. Isolation and Identification of Bovine Viral Diarrhea Virus JN Strain and Sequence Analysis on E2 Gene[J]. , 2013, (5): 83-86.

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Isolation and Identification of Bovine Viral Diarrhea Virus JN Strain and Sequence Analysis on E2 Gene

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