绿脓杆菌焦磷酸测序检测方法的建立

Abstract

Abstract: In this study, we reported a new method of pyrosequencing-based sequence analysis to detect Pseudomonas aeruginosa,which was a rapid simple method. After extracting DNA from cultured cells, Pseudomonas aeruginosa were preliminarily determined by PCR on a specific sequence of the exotoxin A gene which containing conserved fragment respectively. Then the results obtained by PCR were further validated via the pyrosequencing method, and the sequences were demonstrated to specific of Pseudomonas aeruginosa. By conventional sequencing, the sequences results were 100% in agreement with pyrosequencing. Comparing the sequence got by pyrosequencing with the known sequence, you would find the sequence was Pseudomonas aeruginosa.This method was accurate,fast,and could be used efficiently for identifying the Pseudomonas aeruginosa.

Key words: Pseudomonas aeruginosa; PCR; pyrosequencing; identification

CLC Number:

S816.79

ZHANG Tai-xiang, SUN Jun, ZHAO Han, LI Xiu-yong, SUN Tao, LIU Wen-peng, TIAN Guo-ning, HAN Liang. Identification of Pseudomonas aeruginosa Using Pyrosequencing Assays[J]. .

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Identification of Pseudomonas aeruginosa Using Pyrosequencing Assays

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