禽流感免疫噬菌体抗体库构建及ELISA检测方法的建立

Abstract

Abstract: Fab antibody of avian influenza virus subtypes was screened by phage antibody library,and an ELISA detection method was established. Mice were immunized with vaccine mixed of subtypes of AIV, the total RNA was extracted from harvested spleens, immunoglobulin light-chain and heavy-chain gene was amplificated with the template of total RNA, and cloned into phagemid pComb3, the recombinant phagemid pComb3 was transformed into XL1-Blue competent cells, after infected with helper phage VCSM13, Fab antibody library of 8?10⁷ CFU was obtained. Specific clones anti-H5N1 virus was obtained after 4 rounds of screening,ELISA detection method was established with specific clones.

Key words: avian influenza virus; phage antibody library; establishment of detection method

CLC Number:

Q782

XING You-shang, WANG Lin, ZHANG Can, ZHAO Yin-ze, BAI Ya-duo, WU Ya-qiong, LI Yu-xin. Construction of Avian Influenza Immune Phage Antibody Library and Establishment of an ELISA Detection Method[J]. , 2012, 39(6): 17-21.

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Construction of Avian Influenza Immune Phage Antibody Library and Establishment of an ELISA Detection Method

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