一株鸭源H1N6亚型禽流感病毒的分离鉴定及HA和NA基因序列分析

doi:10.16431/j.cnki.1671-7236.2018.02.004

Abstract

Abstract:

In order to understand the epidemic situation of avian influenza virus (AIV) in Sino-Vietnamese border Guangxi, we carried out surveillance of avian influenza in the live poultry market in this region. A strain of H1N6 subtype AIV A/Duck/Guangxi/F01/2016 was isolated from live-bird market in Guangxi-Vietnam border areas. HA and NA genes were sequenced and the genetic analysis of isolate was made with other sequences available in GenBank. The results indicated that HA gene showed 96.9% nucleotide homology with A/sparrow/Guangxi/GXs-1/2012(H1N2), and NA gene showed 98.2% nucleotide homology with A/Pavo cristatus/Jiangxi/JA1/2016(H5N6). The sequence analysis of HA gene showed that the virus was categorized as low pathogenic AIV, with the typical HA gene cleavage site of PSIQSR↓GLF. As the same with other N6 isolates, there were 11 amino acids missing. In addition, the receptor affinity of the isolate was also tested in this study. The result showed that the virus preferentially bind to the sialic acid α-2,3-Gal receptor. These data indicated that A/Duck/Guangxi/F01/2016 could be low pathogenic AIV and recombinant from different subtype of AIV.

Key words: avian influenza virus (AIV); H1N6 subtype; sequence analysis

CLC Number:

S852.65⁺9.6

XIONG Wenjie, XIE Zhixun, LI Meng, CAO Guomin, QIN Hai, YAN Xiaodong. Isolation, Identification and Genetic Analysis of HA and NA Genes of H1N6 Subtype Avian Influenza Virus[J]. , 2018, 45(2): 310-319.

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Isolation, Identification and Genetic Analysis of HA and NA Genes of H1N6 Subtype Avian Influenza Virus

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