2株弱血凝活性H9N2亚型禽流感病毒特性的研究

doi:10.16431/j.cnki.1671-7236.2016.06.034

Abstract

Abstract: To study the characteristics of 2 low hemagglutinating activity H9N2 avian influenza virus (AIV) strains.2 strains were identified by HA/HI,electron microscope observation,HA and NA sequencing.The virus characteristics were studied by morphology,chemistry and biology.2 isolates were identified as H9N2 AIV.The observations demonstrated that the virions expressed various shapes of spherical,oval,elongated forms and polymorphism with diameters in a range of 80 to 120 nm.Hemagglutinin(HA) of the 2 strains was medium-stable to heat,and they were heat-resistant under 50 ℃.2 isolates could agglutinate cock and human O type red blood cells.The agglutination did not appear on the original isolates,but recovered with the passage of virus gradually.2 isolates did not agglutinate pig,cattle,sheep,rabbit and horse red blood cells.2 strains were not sensitive to the ether and chloroform,and only one isolate was sensitive to acids.2 strains were not fatal to the chicken embryo,and the EID₅₀ were both 1×10^-7/0.2 mL.The small White mice showed no clinical symptoms and no deaths after being inoculated with the viruses via respiratory route in our experiment.Serum antibody HI titers of the survived mice were measured after inoculated 14 d,and the antibody of H9N2 AIV were positive.Some tolerant H9N2 strains existed in live animal markets of Shanghai area.

Key words: H9N2 subtype; avian influenza virus; low hemagglutinating activity; virus characteristics

CLC Number:

S852.65

LIU Jian, YANG Xian-chao, XU Feng, LI Xin, DENG Bo, YANG De-quan, JU Hou-bin, QI Xin-yong, GE Jie, ZHOU Jin-ping. Study on the Characteristics of Two Low Hemagglutinating Activity H9N2 AIV Strains[J]. , 2016, 43(6): 1624-1629.

References

[1] 王英珍,李秀丽,鄢明华,等.H9N2亚型禽流感病毒TJ₁株的分离与鉴定[J].动物医学进展,2007,28(10):44-47.
[2] 刘铀,曹永长,毕英佐,等.鸭源H9亚型禽流感病毒的分离与鉴定[J].畜牧与兽医,2006,38(4):48-49.
[3] 黄淑坚,龚中贵,陈秀玲,等.呼吸道病型H9亚型禽流感病毒的分离与鉴定[J].畜牧与兽医,2007,39(3):47-49.
[4] 温纳相,吴德铭,宋延华,等.猪流感病毒的分离与鉴定[J].畜牧与兽医,2008,29(4):53-55.
[5] 黄胜斌,蒋家霞,何奇松,等.广西野鸟H9N2亚型流感病毒的分离与鉴定[J].南方农业学报,2011,42(5):539-543.
[6] 黄良宗,顾万军,王淑敏,等.H3亚型猪流感病毒分离与鉴定[J].动物医学进展,2006,27(6):61-63.
[7] 黄淑坚,龚中贵,吴志新,等.繁殖障碍型H3亚型猪流感病毒的分离与鉴定[J].广东农业科学,2007,7:78-80.
[8] 许传田,赵宏坤,范伟兴,等.H9N2亚型猪流感病毒山东分离株的分离鉴定及其HA、NP、NS基因序列分析[J].中国兽医学报,2004,24(6):528-530.
[9] Munir M,Zohari S,Abbas M,et al.Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates[J].Indian J Virol,2013,24(3):342-348.
[10] Sun Y P,Liu J H.H9N2 influenza virus in China:A cause of concern[J].Protein Cell,2015,6(1):18-25.
[11] Li S,Zhou Y F,Zhao Y X,et al.Avian influenza H9N2 seroprevalence among pig population and pig farm staff in Shandong,China[J].Virology Journal,2015,34(12):1-6.
[12] 张发明,郑杰,王文泉,等.草鸡源H9亚型禽流感病毒的分离与鉴定[J].中国畜牧兽医,2011,38(6):175-178.
[13] Wu R,Sui Z W,Zhang H B,et al.Characterization of a pathogenic H9N2 influenza A virus isolated from central China in 2007[J].Arch Virol,2008,153:1549-1555.
[14] Zhang H B,Xu B,Chen Q J,et al.Characterization of H9N2 influenza viruses isolated from Dongting Lake wetland in 2007[J].Arch Virol,2011,156:95-105.
[15] 程晓霞,朱小丽,王劭,等.H9亚型禽流感病毒的分离与鉴定[J].现代农业科技,2009,13:303-305.
[16] Zhu Y B,Yang Y,Liu W,et al.Comparison of biological characteristics of H9N2 avian influenza viruses isolated from different hosts[J].Arch Virol,2015,160:917-927.
[17] Deng G C,Bi J M,Kong F L,et al.Acute respiratory distress syndrome induced by H9N2 virus in mice[J].Arch Virol,2010,155:187-195.

Study on the Characteristics of Two Low Hemagglutinating Activity H9N2 AIV Strains

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[2]	XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1319-1328.
[3]	HOU Xiaohong, WANG Meng, WU Bo, LONG Xianrong, ZHAO Xiaomin, GAO Jing, DAI Peiqiang, CHAI Tongjie. Infectivity of H9N2 Subtype Avian Influenza Virus SD18 Strain and Comparison of the Effects of Different Routes of Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1449-1460.
[4]	YANG Jing, ZHANG Xinyu, LIANG Zhipeng, CHENG Qing, WANG Congying, CHI Shihong, YUAN Sheng, GUO Jinyue, HUANG Shujian, WEN Feng. Prokaryotic Expression of NP Protein of H9N2 Subtype Avian Influenza Virus and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3963-3971.
[5]	JIANG Ning, YIN Hang, ZHANG Yanwei, LI Zixin, CHI Xiaojuan, WANG Song. Research Progress on Co-infection of H9N2 Subtype Avian Influenza Virus with Other Pathogens [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3447-3455.
[6]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.
[7]	CHENG Dengfang, MEI Chen, LIU Juan, WANG Hongjun, XIAN Hong. Effect of Chinese Herbal Prescription on the Proliferation of H9N2 in MDCK Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 650-657.
[8]	LI Qingmei, GUO Junqing, MENG Zekun, LI Yanhua, LIU Xiao, SHI Jianzhou, LI Ge, CHAI Shujun, LUO Jun, DENG Ruiguang, ZHANG Gaiping. Preparation and Application of Neutralizing Monoclonal Antibodies Against H9N2 Subtype Avian Influenza Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3761-3769.
[9]	CHENG Yi, TIAN Hui, ZHANG Ya, XUE Jingjing, SHENG Dongbei, LIU Wujie, WANG Wanbing, ZHANG Pantao, TIAN Kegong. Isolation,Identification and Immunogenicity Evaluation of H9N2 Subtype Avian Influenza Virus HF Strain [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2945-2952.
[10]	LI Jiajia, LI Suhua, SONG Jianling, HUANG Jingjun, MIAO Yuan, CHEN Xi, JI Jia. Sequence Analysis of HA and NA Genes of Two H9N2 Subtype Avian Influenza Virus in Yunnan [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 873-883.
[11]	PENG Chengcheng, WU Yixiao, LIU Xuping, TAN Wensong. Study on Methods for Purification of H9N2 Subtype Avian Influenza Virus and Quantification of Viral HA Protein [J]. , 2019, 46(7): 2069-2078.
[12]	LI Peiyao, LIANG Ting, LUO Qiang, WANG Shaohua, LI Jun, XU Mingju, ZHANG Ruihua, XU Tong. Study on Optimal Proliferation Conditions of H9N2 Subtype Swine Influenza Virus in Mouse Pulmonary Microvascular Endothelial Cells [J]. , 2018, 45(6): 1708-1714.
[13]	XIONG Wenjie, XIE Zhixun, LI Meng, CAO Guomin, QIN Hai, YAN Xiaodong. Isolation, Identification and Genetic Analysis of HA and NA Genes of H1N6 Subtype Avian Influenza Virus [J]. , 2018, 45(2): 310-319.
[14]	CHENG Dengfang, WANG Hongjun, HUANG Qingzhou, ZHANG Li, LIU Juan, XIAN Hong. Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo [J]. , 2018, 45(12): 3594-3600.
[15]	WAN Run, HUA Min, LONG Lishu, HE Xinwei, LUO Yinxing, ZHOU Bijun, WANG Kaigong, CHENG Zhentao, WEN Ming. Prokayotic Expression and Polyclonal Antibody Preparation of N6 Gene of H6N6 Subtype Avian Influenza Virus [J]. , 2018, 45(11): 2989-2995.