中药复方对H9N2亚型禽流感病毒在MDCK细胞增殖的作用

doi:10.16431/j.cnki.1671-7236.2021.02.027

Abstract

Abstract: To screen traditional Chinese medicines to prevent and treat viral disease H9N2 Avian influenza virus (AIV),Qingen extract (QG) was used to evaluate its anti-H9N2 efficiency through the neuraminidase (NA) inhibition and CCK-8 method from the aspects of virus adsorption,proliferation and inactivation,with oseltamivir (Tamiflu,DF) as positive drug control,based on AIV clinical symptoms and basic principles of traditional Chinese medicine.The results showed that the inhibitory effect of QG on NA increased in concentration dependent manner,and IC₅₀ was about 1 130 μg/mL.The maximum safety concentrations of QG and DF were 2 500 μg/mL and 500 μg/mL respectively,and the TCID₅₀ of H9N2 AIV was 10^-4.36/100 μL.Among the concentration range of 156.25-2 500 μg/mL,QG weaken the virulence of H9N2 AIV to a certain extent,and showed anti-attachment and anti-viral replication effects on MDCK cells.In conclusion,QG could inhibit the NA activity of H9N2 AIV,inhibit H9N2 AIV's attachment and replication to target cells.

Key words: Avian influenza virus; H9N2; Chinese herbal prescription; MDCK cells

CLC Number:

S853.7

CHENG Dengfang, MEI Chen, LIU Juan, WANG Hongjun, XIAN Hong. Effect of Chinese Herbal Prescription on the Proliferation of H9N2 in MDCK Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 650-657.

References

[1] RABEH E S,JOHN F,MARATHE B M,et al.Genetic characterization and pathogenic potential of H10 avian influenza viruses isolated from live poultry markets in Bangladesh[J].Scientific Reports,2018,8(1):10693.
[2] KWON J S,LEE H J,LEE D H,et al.Immune responses and pathogenesis in immunocompromised chickens in response to infection with the H9N2 low pathogenic avian influenza virus[J].Virus Research,2008,133(2):187-194.
[3] PANTIN-JACKWOOD M J,SWAYNE D E.Pathogenesis and pathobiology of avian influenza virus infection in birds[J].Revue Scientifique et Technique,2009,28(1):113-136.
[4] GU M,XU L,WANG X,et al.Current situation of H9N2 subtype avian influenza in China[J].Veterinary Research,2017,48:49.
[5] WU Y,LIN J,YANG S,et al.The molecular characteristics of avian influenza viruses (H9N2) derived from air samples in live poultry markets[J].Infection,Genetics and Evolution,2018,60:191-196.
[6] LI Y,LIU M,SUN Q,et al.Genotypic evolution and epidemiological characteristics of H9N2 influenza virus in Shandong Province,China[J].Poultry Science,2019,98(9):3488-3495.
[7] TONG S X,ZHU X Y,LI Y,et al.New world bats harbor diverse influenza A viruses[J].PLoS Pathogens,2013,9(10):e1003657.
[8] PAWAR S D,TANDALE B V,RAUT C G,et al.Avian influenza H9N2 seroprevalence among poultry workers in Pune,India 2010[J].PLoS One,2012,7(10):1371.
[9] CUI L,LIU D,SHI W,et al.Dynamic reassortments and genetic hetetogeneity of human-infecting influnenza (H7N9) virus[J].Nature Communications,2014,5:3142-3149.
[10] 辛丽,白天,周剑芳,等.中国职业暴露人群感染H9N2禽流感病毒血清学调查[J].疾病监测,2015,30(5):368-371. XIN L,BAI T,ZHOU J F,et al.Serological study of human infection with avian influenza A (H9N2) virus in population with occupational exposure in China[J].Disease Surveillance,2015,30(5):368-371.(in Chinese)
[11] LIU Y F,LAI H Z,LIN L,et al.Endemic variation of H9N2 avian influenza virus in China[J].Avian Diseases,2016,60:817-825.
[12] YU Q,LIU L,PU J,et al.Risk perceptions for avian influenza virus infection among poultry workers,China[J].Emerging Infectious Diseases,2013,19(2):313-316.
[13] LIU Q,LIU Y,YANG J,et al.Two genetically similar H9N2 influenza A viruses show different pathogenicity in mice[J].Frontiers in Microbiology,2016,7:1737.
[14] 殷震,刘景华.动物病毒学[M].北京:科学出版社,1997. YIN Z,LIU J H.Animal Virology[M].Beijing:Science Press,1997.(in Chinese)
[15] RIJAL P,WANG B B,TAN T K,et al.Broadly inhibiting anti-neuraminidase monoclonal antibodies induced by 2 trivalent influenza vaccine and H7N9 infection in humans[J].Journal of Virology,2020,94(4):e01182-19.
[16] CHOI W S,JEONG J H,KWON J J,et al.Screening for neuraminidase inhibitor resistance markers among avian influenza viruses of the N4,N5,N6,and N8 neuraminidase subtypes[J].Journal of Virology,2017,92(1):e01580-17.
[17] 蔡文涛.MTT法和CCK-8法检测中药抗病毒活性成分细胞毒性的比较[J].湖北大学学报(自然科学版),2017,39(3):305-310. CAI W T.The comparision of MTT and CCK-8 by detecting cytotoxicity of antiviral active components in traditional Chinese medicine[J].Journal of Hubei University (Natural Science),2017,39(3):305-310.(in Chinese)
[18] YU Y N,ZHENG Y,HAO S S,et al.The molecular evolutionary characteristics of new isolated H9N2 AIV from East China and the function of vimentin on virus replication in MDCK cells[J].Journal of Virology,2020,17(1):78.
[19] 王思源,平静,谢宁,等.穿心莲内酯磺化物体外抗流感病毒药效学研究[J].中南药学,2013,11(5):331-334. WANG S Y,PING J,XIE N,et al.In vitro pharmacodynamics effect of andrographolide sulfonated on anti-infuenza viruses[J].Centrai South Pharmacy,2013,11(5):331-334.(in Chinese)
[20] SANPHA K,LI X R,JI J,et al.Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo[J].Journal of Animal Science and Biotechnology,2013,4:22-37.

Effect of Chinese Herbal Prescription on the Proliferation of H9N2 in MDCK Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[2]	XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1319-1328.
[3]	ZHANG Yuan, YU Mengmeng, SUN Liuke, GUO Xing, NA Lei, LIU Diqiu, JIANG Qian, ZHANG Haili, WANG Xiaojun. Analysis of Canine ANP32 Protein Polymorphism and Effect on RNA Polymerase Activity of Influenza A Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2462-2473.
[4]	HOU Xiaohong, WANG Meng, WU Bo, LONG Xianrong, ZHAO Xiaomin, GAO Jing, DAI Peiqiang, CHAI Tongjie. Infectivity of H9N2 Subtype Avian Influenza Virus SD18 Strain and Comparison of the Effects of Different Routes of Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1449-1460.
[5]	YANG Jing, ZHANG Xinyu, LIANG Zhipeng, CHENG Qing, WANG Congying, CHI Shihong, YUAN Sheng, GUO Jinyue, HUANG Shujian, WEN Feng. Prokaryotic Expression of NP Protein of H9N2 Subtype Avian Influenza Virus and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3963-3971.
[6]	JIANG Ning, YIN Hang, ZHANG Yanwei, LI Zixin, CHI Xiaojuan, WANG Song. Research Progress on Co-infection of H9N2 Subtype Avian Influenza Virus with Other Pathogens [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3447-3455.
[7]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.
[8]	LI Qingmei, GUO Junqing, MENG Zekun, LI Yanhua, LIU Xiao, SHI Jianzhou, LI Ge, CHAI Shujun, LUO Jun, DENG Ruiguang, ZHANG Gaiping. Preparation and Application of Neutralizing Monoclonal Antibodies Against H9N2 Subtype Avian Influenza Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3761-3769.
[9]	CHENG Yi, TIAN Hui, ZHANG Ya, XUE Jingjing, SHENG Dongbei, LIU Wujie, WANG Wanbing, ZHANG Pantao, TIAN Kegong. Isolation,Identification and Immunogenicity Evaluation of H9N2 Subtype Avian Influenza Virus HF Strain [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2945-2952.
[10]	LI Jiajia, LI Suhua, SONG Jianling, HUANG Jingjun, MIAO Yuan, CHEN Xi, JI Jia. Sequence Analysis of HA and NA Genes of Two H9N2 Subtype Avian Influenza Virus in Yunnan [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 873-883.
[11]	PENG Chengcheng, WU Yixiao, LIU Xuping, TAN Wensong. Study on Methods for Purification of H9N2 Subtype Avian Influenza Virus and Quantification of Viral HA Protein [J]. , 2019, 46(7): 2069-2078.
[12]	LI Peiyao, LIANG Ting, LUO Qiang, WANG Shaohua, LI Jun, XU Mingju, ZHANG Ruihua, XU Tong. Study on Optimal Proliferation Conditions of H9N2 Subtype Swine Influenza Virus in Mouse Pulmonary Microvascular Endothelial Cells [J]. , 2018, 45(6): 1708-1714.
[13]	XIONG Wenjie, XIE Zhixun, LI Meng, CAO Guomin, QIN Hai, YAN Xiaodong. Isolation, Identification and Genetic Analysis of HA and NA Genes of H1N6 Subtype Avian Influenza Virus [J]. , 2018, 45(2): 310-319.
[14]	CHENG Dengfang, WANG Hongjun, HUANG Qingzhou, ZHANG Li, LIU Juan, XIAN Hong. Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo [J]. , 2018, 45(12): 3594-3600.
[15]	WAN Run, HUA Min, LONG Lishu, HE Xinwei, LUO Yinxing, ZHOU Bijun, WANG Kaigong, CHENG Zhentao, WEN Ming. Prokayotic Expression and Polyclonal Antibody Preparation of N6 Gene of H6N6 Subtype Avian Influenza Virus [J]. , 2018, 45(11): 2989-2995.