黑色素皮质激素受体1基因248位点多态性与中国马品种毛色的相关性分析

Abstract

Abstract: A single nucleotide polymorphism (SNP) at site 248 of melanocortin receptor 1 gene (MC1R) is known from European horse breeds, in which the homozygous genotype (ee) is related with the chestnut coat color of European horse individuals. To confirm the polymorphism of site 248 of MC1R gene in Chinese horse, three horse breeds of Guizhou pony, Southwest horse and Ili horse were chosen as samples with coat color of three monochromatic (chestnut, black, bay) and three kinds of complex coat color. Two pairs specific primers were designed. Two fragments, 124 and 481 bp, were amplificated by allele-specific polymerase chain reaction (AS-PCR) method. The polymorphism of site 248 in MC1R gene was further approved by cloning and sequencing of the DNA fragments. The results showed that genotypes of all samples with coat color of chestnut were heterozygous genotype (Ee). However, the genotypes of other samples were Ee either, in which the coat colors included bay, black, grey, spotted but not chestnut. It suggested that the SNP at site 248 of MC1R gene might be unrelated with the chestnut coat color in native Chinese horse breeds.

Key words: Guizhou pony; Southwest horse; Ili horse; coat color; MC1R gene

CLC Number:

S821

DANG Zhen, WANG Jia-fu, ZHAO Xing-yan, HUANG Yong, TIAN Song-jun, WANG Rong-ming, RAN Xue-qin. Association of Polymorphism in MC1R Gene at Site 248 with Chestnut Coat Color in Three Chinese Horse Breeds[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(6): 149-152.

References

1 于云柱,何奕多,刘丽,等. 黑素皮质素受体1(MC1R)基因的研究进展[J].中国畜牧兽医,2010,37(6):232~233.
2 甘肃农业大学.养马学[M].北京:农业出版社,1981,242.
3 甘海云,李建斌,王洪梅,等.牛黑素皮质素受体Ⅰ(MC1R)基因与毛色表型的研究[J].遗传,2007,29(2):195~200.
4 孙玉江,闵令江,陈建兴,等.中国西南马群体遗传资源特征分析[J].华北农学报,2009,24(2):94~98.
5 汤灵姿,欧阳文,谭小海,等.伊犁马主要体尺性状测定及分析[J].新疆农业科学,2007,44(5):691~695.
6 Lauvergne J J, Silvestrelli M, Langlois B, et al. A new schemefor describing horse coat colour. Livest Prod Sci, 1991, 27:21~229.
7 Jackson I J. Colour-coded switches. Nature, 1993, 362: 587~588.
8 Jackson I J. Homologous pigmentation mutations in human, mouse and other model organisms. Hum Mol Genet, 1997, 6: 1613~1624.
9 Johansson M, Marklund L, Sandberg K, et al. Cosegregation between the chestnut coat colour in horses and polymorphisms at the melanocyte stimulating hormone (MSH) receptor locus. Anim Genet, 1994, 25: 35.
10 Klungland H, Vage D I, Gomez-Raya L, et al. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm Genome, 1995, 6: 636~639.
11 Marklund L, Moiler J M, Sandberg K, et al. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses. Mamm Genome, 1996, 7: 895~899.
12 Robbins L S, Nadeau J H, Johnson K R, et al. Pigmentation phenotypes of variant extension locus alleles result from point mutations that alter MSH receptor function. Cell, 1993, 72: 827~834.
13 Thiruvenkadan A K, Kandasamy N, Panneerselvam S. Coat colour inheritance in horses. Livest Sci, 2008, 117: 109~129.
14 Valverde P, Healy E, Jackson I, et al. Variants of the melanocyte-stimulating hormone receptor gene are associated with red hair and fair skin in humans. Nature Genet, 1995, 11: 328~330.

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Association of Polymorphism in MC1R Gene at Site 248 with Chestnut Coat Color in Three Chinese Horse Breeds

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