卵泡刺激素β基因编码区SNPs与糯谷猪繁殖性能的关联性分析

›› 2012, Vol. 39 ›› Issue (5): 148-151.

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Association Analyses of Single Nucleotide Polymorphism of FSHβ Gene on Reproductive Performance in Nuogu Pig

CHU Wei-wei¹, GAO Ying², LIN Jia-dong¹, YAN Lei¹, PAN Lan-bing¹, LI Pei-li¹

1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;2. Laboratory for Amimal Disease of Guizhou, College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2011-11-23 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

Abstract

Abstract: This study aimed to investigate molecular genetic characteristics of the follicle-stimulating hormone (FSH) β gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on some reproductive performance in Nuogu pig. We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing the coding sequence of the FSHβ gene in 58 individuals. The results showed that two new polymorphic sites were found in exon 2 on 6699 bp and 6708 bp. The A allele frequency was 0.288,the B allele frequency was 0.119,while the C allele frequency was 0.593. There were no significant difference in five genotypes(AA,CC,AB,AC,BC),because of the two mutant sites were the synonymy mutations.

Key words: Nuogu pig; FSHβ gene; exon; PCR-SSCP; SNPs

CLC Number:

S813

CHU Wei-wei;GAO Ying;LIN Jia-dong;YAN Lei;PAN Lan-bing;LI Pei-li. Association Analyses of Single Nucleotide Polymorphism of FSHβ Gene on Reproductive Performance in Nuogu Pig[J]. , 2012, 39(5): 148-151.

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Association Analyses of Single Nucleotide Polymorphism of FSHβ Gene on Reproductive Performance in Nuogu Pig

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