奶牛乳腺上皮细胞核蛋白质组双向电泳方法的建立

›› 2012, Vol. 39 ›› Issue (2): 31-34.

• 生物技术 • Previous Articles Next Articles

Establishment of Two-dimensional Electrophoresis Method of Bovine Mammary Epithelial Cell Nucleus Proteomics

LIU Rong, GAO Xue-jun, LI Qing-zhang, HUANG Jian-guo, LU Li-min, LUO Chao-chao, JIN Xin, WANG Jia-li

The Key Laboratory of Dairy Science of Education, Northeast Agricultural University, Harbin 150030, China

Received:2011-09-08 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

Abstract

Abstract: The resolution of 2-DE map is highly altered due to different experimental conditions, a 2-DE map with high resolution can be obtained via the optimization of key experimental conditions for a definite proteome sample. To establish an effective separation system of 2-DE for the proteome of bovine mammary epithelial cell nucleus, 2-DE method was researched on the standard experimental condition with an optimization for each important experimental parameter. The numbers of protein spots, resolution and contaminating strands in 2-DE maps were determined. A 2-DE separation system for proteome of bovine mammary epithelial cell nucleus proteins was successfully established.

Key words: 2-DE; proteomics; bovine mammary epithelial cell; cell nucleus proteins

CLC Number:

LIU Rong;GAO Xue-jun;LI Qing-zhang;HUANG Jian-guo;LU Li-min;LUO Chao-chao;JIN Xin;WANG Jia-li. Establishment of Two-dimensional Electrophoresis Method of Bovine Mammary Epithelial Cell Nucleus Proteomics[J]. , 2012, 39(2): 31-34.

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Establishment of Two-dimensional Electrophoresis Method of Bovine Mammary Epithelial Cell Nucleus Proteomics

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